Overview

  • Product name

  • Description

    Rabbit polyclonal to LeuRS
  • Host species

    Rabbit
  • Specificity

    This antibody reacts specifically with LeuRS.

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human LeuRS.

  • General notes

     This product was previously labelled as Leucyl tRNA synthetase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab31534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 135 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/200.

Target

  • Function

    Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.
  • Involvement in disease

    Infantile liver failure syndrome 1
  • Sequence similarities

    Belongs to the class-I aminoacyl-tRNA synthetase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • cytoplasmic antibody
    • Cytoplasmic leucyl tRNA synthetase antibody
    • Cytosolic leucyl tRNA synthetase antibody
    • FLJ10595 antibody
    • FLJ21788 antibody
    • hr025Cl antibody
    • HSPC 192 antibody
    • HSPC192 antibody
    • KIAA1352 antibody
    • LARS 1 antibody
    • Lars antibody
    • LARS1 antibody
    • Leucine translase antibody
    • Leucine tRNA ligase antibody
    • Leucine--tRNA ligase antibody
    • Leucyl tRNA synthetase cytoplasmic antibody
    • Leucyl-tRNA synthetase antibody
    • LeuRS antibody
    • LEUS antibody
    • LRS antibody
    • PIG 44 antibody
    • PIG44 antibody
    • Proliferation inducing gene 44 antibody
    • RNTLS antibody
    • SYLC_HUMAN antibody
    see all

Images

  • All lanes : Anti-LeuRS antibody (ab31534) at 1/4000 dilution

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa

  • ab31534 used in Western Blot at a 1/4,000 dilution.
  • ab31534 at a 1/200 dilution staining LeuRS in human colon cancer tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
  • Su X  et al. Interferon-? regulates cellular metabolism and mRNA translation to potentiate macrophage activation. Nat Immunol 16:838-849 (2015). Read more (PubMed: 26147685) »
See all 6 Publications for this product

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There are currently no Customer reviews or Questions for ab31534.
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