Key features and details
- Rabbit polyclonal to LexA DNA Binding Region
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-LexA DNA Binding Region antibody
See all LexA DNA Binding Region primary antibodies
DescriptionRabbit polyclonal to LexA DNA Binding Region
SpecificityRecognizes the LexA portion of bait fusion proteins used in the yeast two-hybrid system.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Species independent
Fusion protein (His-tag) corresponding to Escherichia coli LexA DNA Binding Region aa 1-202. Immunogen is identical for SwissProt: P0A7C2, P0A7C3, P0A7C4 His-tagged DNA binding domain of LexA protein corresponding to aa1-202
MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKAL ARKGVIEIVS GASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHY QVDPSLFKPNADFLLRVSGMSM KDIGIMDGDLLAVHKTQDVRNGQVVV ARIDDEVTVKRLKKQGNKVELLPENSEFKPIVVD LRQQSFTIEGLAVG VIRNGDWL
Database link: P0A7C2
- LexA-pRB-transfected yeast cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 0.87% Sodium chloride
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab14553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 µg/ml. Predicted molecular weight: 49 kDa.
Detects LexA-pRB in LexA-pRB transfected yeast cells.
|IP||Use a concentration of 0.1 µg/ml.
5ug immunoprecipitates LexA-pRB from 500ug LexA-pRB transfected yeast cells.
RelevanceThe LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
- ExrA antibody
- Lex A antibody
- LexA antibody
ab14553 has been referenced in 10 publications.
- Paredes-Amaya CC et al. The Hcp-like protein HilE inhibits homodimerization and DNA binding of the virulence-associated transcriptional regulator HilD in Salmonella. J Biol Chem 293:6578-6592 (2018). PubMed: 29535187
- Vilaboa N et al. New inhibitor targeting human transcription factor HSF1: effects on the heat shock response and tumor cell survival. Nucleic Acids Res 45:5797-5817 (2017). WB ; Human . PubMed: 28369544
- Zhang T et al. Mapping ubiquitination sites of S. cerevisiae Mcm10. Biochem Biophys Rep 8:212-218 (2016). PubMed: 28497125
- Rother S et al. NF-?B-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'?3' exoribonuclease 2 and negative elongation factor. FASEB J 30:174-85 (2016). PubMed: 26340924
- Dinesh DC et al. Solution structure of the PsIAA4 oligomerization domain reveals interaction modes for transcription factors in early auxin response. Proc Natl Acad Sci U S A 112:6230-5 (2015). PubMed: 25918389
- Alver RC et al. The N-terminus of Mcm10 is important for interaction with the 9-1-1 clamp and in resistance to DNA damage. Nucleic Acids Res N/A:N/A (2014). WB . PubMed: 24972833
- Guadarrama C et al. The Salmonella enterica Serovar Typhi LeuO Global Regulator Forms Tetramers: Residues Involved in Oligomerization, DNA Binding, and Transcriptional Regulation. J Bacteriol 196:2143-54 (2014). WB . PubMed: 24659766
- Sellamuthu S et al. An engineered viral protease exhibiting substrate specificity for a polyglutamine stretch prevents polyglutamine-induced neuronal cell death. PLoS One 6:e22554 (2011). WB . PubMed: 21799895
- Wang X et al. Proteolytic instability and the action of nonclassical transcriptional activators. Curr Biol 20:868-71 (2010). PubMed: 20417106
- Sardi SP et al. Presenilin-dependent ErbB4 nuclear signaling regulates the timing of astrogenesis in the developing brain. Cell 127:185-97 (2006). PubMed: 17018285