Key features and details
- Rabbit polyclonal to LexA DNA Binding Region
- Suitable for: WB, IP, ChIP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-LexA DNA Binding Region antibody
See all LexA DNA Binding Region primary antibodies
DescriptionRabbit polyclonal to LexA DNA Binding Region
Tested applicationsSuitable for: WB, IP, ChIP, ICC/IFmore details
Species reactivityReacts with: Species independent
Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region.
Database link: POA7C2
- E. coli whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Preservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab174384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/3000. Predicted molecular weight: 22 kDa.|
|IP||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceThe LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
- ExrA antibody
- Lex A antibody
- LexA antibody
ab174384 has been referenced in 1 publication.
- Hishida T et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev 18:1886-97 (2004). WB ; Escherichia coli . PubMed: 15289460