Anti-LexA DNA Binding Region antibody (ab174384)
Key features and details
- Rabbit polyclonal to LexA DNA Binding Region
- Suitable for: WB, IP, ChIP, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-LexA DNA Binding Region antibody
See all LexA DNA Binding Region primary antibodies -
Description
Rabbit polyclonal to LexA DNA Binding Region -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIP, ICC/IFmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region.
Database link: POA7C2 -
Positive control
- E. coli whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/3000. Predicted molecular weight: 22 kDa.
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IP |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/3000. Predicted molecular weight: 22 kDa. |
IP
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 1039346 Escherichia coli
- Entrez Gene: 948544 Escherichia coli
- Entrez Gene: 960080 Escherichia coli
- SwissProt: P0A7C2 Escherichia coli
- SwissProt: P0A7C3 Escherichia coli
- SwissProt: P0A7C4 Escherichia coli
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Alternative names
- ExrA antibody
- Lex A antibody
- LexA antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab174384 has been referenced in 4 publications.
- Hughes KJ et al. An RNA Repair Operon Regulated by Damaged tRNAs. Cell Rep 33:108527 (2020). PubMed: 33357439
- Kramarz K et al. The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal. Nat Commun 11:5643 (2020). PubMed: 33159083
- Sun Y et al. Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic Escherichia coli and Contributes to Opsonophagocytosis Resistance in Bacteria. Front Cell Infect Microbiol 10:592906 (2020). PubMed: 33569353
- Hishida T et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev 18:1886-97 (2004). WB ; Escherichia coli . PubMed: 15289460