Key features and details
- Rabbit polyclonal to LexA DNA Binding Region
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-LexA DNA Binding Region antibody
See all LexA DNA Binding Region primary antibodies
DescriptionRabbit polyclonal to LexA DNA Binding Region
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Escherichia coli, Shigella flexneri
Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region aa 1-202.
MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKAL ARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQV DPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARID DEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGD WL
Database link: P0A7C2
- WB: Recombinant Escherichia coli LexA DNA Binding Region protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab234695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
RelevanceThe LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
- ExrA antibody
- Lex A antibody
- LexA antibody
All lanes : Anti-LexA DNA Binding Region antibody (ab234695) at 1/500 dilution
Lane 1 : Recombinant Escherichia coli LexA Binding Region protein, 80 ng
Lane 2 : Recombinant Escherichia coli LexA Binding Region protein, 40 ng
Lane 3 : Recombinant Escherichia coli LexA Binding Region protein, 20 ng
Lane 4 : Recombinant Escherichia coli LexA Binding Region protein, 10 ng
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234695 has not yet been referenced specifically in any publications.