Product nameAnti-LexA DNA Binding Region antibody
See all LexA DNA Binding Region primary antibodies
DescriptionRabbit polyclonal to LexA DNA Binding Region
Tested applicationsSuitable for: WBmore details
Predicted to work with: Escherichia coli, Shigella flexneri
Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region aa 1-202.
MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKAL ARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQV DPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARID DEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGD WL
Database link: P0A7C2
- WB: Recombinant Escherichia coli LexA DNA Binding Region protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab234695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
RelevanceThe LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
- ExrA antibody
- Lex A antibody
- LexA antibody
All lanes : Anti-LexA DNA Binding Region antibody (ab234695) at 1/500 dilution
Lane 1 : Recombinant Escherichia coli LexA Binding Region protein, 80 ng
Lane 2 : Recombinant Escherichia coli LexA Binding Region protein, 40 ng
Lane 3 : Recombinant Escherichia coli LexA Binding Region protein, 20 ng
Lane 4 : Recombinant Escherichia coli LexA Binding Region protein, 10 ng
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
ab234695 has not yet been referenced specifically in any publications.