Overview

  • Product name

    Anti-LGALS14 antibody [EPR9665]
    See all LGALS14 primary antibodies
  • Description

    Rabbit monoclonal [EPR9665] to LGALS14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LGALS14 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human LGALS14 protein (ab101087) can be used as a positive control in WB. Human placenta lysate; Mouse placenta lysate; JAR cells. ICC/IF: JAR cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9665
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/20.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.
    • Tissue specificity

      Highly expressed in placenta.
    • Sequence similarities

      Contains 1 galectin domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Charcot Leyden crystal protein 2 antibody
      • Charcot-Leyden crystal protein 2 antibody
      • CLC2 antibody
      • Gal 14 antibody
      • Gal-14 antibody
      • Gal14 antibody
      • Galectin 14 antibody
      • Galectin-14 antibody
      • Galectin14 antibody
      • Lectin galactoside binding soluble 14 antibody
      • LGALS14 antibody
      • MGC22235 antibody
      • Placental protein 13 like antibody
      • Placental protein 13 like protein antibody
      • Placental protein 13-like antibody
      • PPL13 antibody
      • PPL13_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution (Purified) + Human placenta lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 16 kDa
      Observed band size: 16 kDa

    • Flow Cytometry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution ((unpurified)) + Human placenta lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 16 kDa

    • Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution ((unpurified)) + Mouse placenta lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 16 kDa

    • Immunofluorescent analysis of JAR cells labelling LGALS14 with unpurified ab150427 at 1/100 dilution.

    References

    ab150427 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150427.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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