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Anti-LGALS3BP antibody (ab67353)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (4)

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Western blot - Anti-LGALS3BP antibody (ab67353)

    Key features and details

    • Mouse polyclonal to LGALS3BP
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-F4/80 antibody (ab100790)
    Protein
    Product image
    Recombinant Human LGALS3BP protein (ab132454)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-LGALS3BP antibody
      See all LGALS3BP primary antibodies
    • Description

      Mouse polyclonal to LGALS3BP
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Full length protein, corresponding to amino acids 1-585 of Human LGALS3BP

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.4
      Constituent: 2.68% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cancer
      • Cell cycle
      • Cell division
      • Cancer
      • Tumor biomarkers
      • Oncoproteins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)
    • Recombinant Protein

      • Recombinant Human LGALS3BP protein (ab132454)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab67353 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (2)
    1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 65 kDa).
    Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 65 kDa).

    Target

    • Function

      Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
    • Tissue specificity

      Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
    • Sequence similarities

      Contains 1 BACK (BTB/Kelch associated) domain.
      Contains 1 BTB (POZ) domain.
      Contains 1 SRCR domain.
    • Cellular localization

      Secreted. Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession Q08380 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3959 Human
      • Omim: 600626 Human
      • SwissProt: Q08380 Human
      • Unigene: 514535 Human
      • Alternative names

        • 90K antibody
        • 90K MAC 2 BP antibody
        • Basement membrane autoantigen p105 antibody
        • BTBD17B antibody
        • Galectin 3 binding protein antibody
        • Galectin-3-binding protein antibody
        • L3 antigen antibody
        • Lectin galactoside binding soluble 3 binding protein antibody
        • Lectin galactoside-binding soluble 3-binding protein antibody
        • LG3BP_HUMAN antibody
        • LGALS3BP antibody
        • M2BP antibody
        • Mac 2 binding protein antibody
        • Mac 2 BP antibody
        • Mac-2 BP antibody
        • Mac-2-binding protein antibody
        • Mac2 BP antibody
        • MAC2BP antibody
        • Serum protein 90K antibody
        • TANGO10B antibody
        • Transport and golgi organization 10 homolog B antibody
        • Tumor associated antigen 90K antibody
        • Tumor-associated antigen 90K antibody
        see all

      Images

      • Western blot - Anti-LGALS3BP antibody (ab67353)
        Western blot - Anti-LGALS3BP antibody (ab67353)
        All lanes : Anti-LGALS3BP antibody (ab67353) at 1/500 dilution

        Lane 1 : LGALS3BP transfected 293T cell lysate
        Lane 2 : Non transfected 293T cell lysate

        Lysates/proteins at 25 µg per lane.

        Secondary
        All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

        Predicted band size: 65 kDa
        Observed band size: 80 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (4)

      Publishing research using ab67353? Please let us know so that we can cite the reference in this datasheet.

      ab67353 has been referenced in 4 publications.

      • Rodríguez-Gallego E  et al. Proteomic Profile Associated With Loss of Spontaneous Human Immunodeficiency Virus Type 1 Elite Control. J Infect Dis 219:867-876 (2019). PubMed: 30312441
      • Kamada Y  et al. Use of Mac-2 binding protein as a biomarker for nonalcoholic fatty liver disease diagnosis. Hepatol Commun 1:780-791 (2017). PubMed: 29404494
      • Kono M  et al. Increased levels of serum Wisteria floribunda agglutinin-positive Mac-2 binding protein in idiopathic pulmonary fibrosis. Respir Med 115:46-52 (2016). PubMed: 27215503
      • Tian Y  et al. Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 11:4677-87 (2011). WB ; Human . PubMed: 22113853

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-LGALS3BP antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (glioma)
      Gel Running Conditions
      Reduced Denaturing (10%)
      Loading amount
      20 µg
      Specification
      glioma
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Xiaoqiang Xia

      Verified customer

      Submitted Apr 08 2016

      Western blot abreview for Anti-LGALS3BP antibody

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Heart, Left ventricle)
      Loading amount
      60 µg
      Specification
      Heart, Left ventricle
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Dawn Bowles

      Verified customer

      Submitted Jan 09 2013

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