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RecombinantRabMAb

Recombinant Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Submit a review Submit a question References (1)
Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
  • Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
  • Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
  • Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
  • Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
  • Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21757-33] to LGALS3BP
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-LGALS3BP antibody [EPR21757-33]
    See all LGALS3BP primary antibodies

  • Description

    Rabbit monoclonal [EPR21757-33] to LGALS3BP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human LGALS3BP aa 1-400. The exact sequence is proprietary.
    Database link: Q08380

  • Positive control

    • WB: Human fetal brain, fetal kidney and heart lysate; HepG2, C6, RAW 264.7, PC-12, NIH/3T3, MCF7, A549, HeLa, 4T1 and Hepa1-6 whole cell lysate; Rat brain, liver and spleen lysate. Mouse heart and kidney lysate. Flow: MCF7 and HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21757-33

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab217572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60.
WB
1/1000. Predicted molecular weight: 65 kDa.
Notes
Flow Cyt (Intra)
1/60.
WB
1/1000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.

  • Tissue specificity

    Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.

  • Sequence similarities

    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 1 SRCR domain.

  • Cellular localization

    Secreted. Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 90K antibody
    • 90K MAC 2 BP antibody
    • Basement membrane autoantigen p105 antibody
    • BTBD17B antibody
    • Galectin 3 binding protein antibody
    • Galectin-3-binding protein antibody
    • L3 antigen antibody
    • Lectin galactoside binding soluble 3 binding protein antibody
    • Lectin galactoside-binding soluble 3-binding protein antibody
    • LG3BP_HUMAN antibody
    • LGALS3BP antibody
    • M2BP antibody
    • Mac 2 binding protein antibody
    • Mac 2 BP antibody
    • Mac-2 BP antibody
    • Mac-2-binding protein antibody
    • Mac2 BP antibody
    • MAC2BP antibody
    • Serum protein 90K antibody
    • TANGO10B antibody
    • Transport and golgi organization 10 homolog B antibody
    • Tumor associated antigen 90K antibody
    • Tumor-associated antigen 90K antibody
    see all

Images

  • Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

    Predicted band size: 65 kDa
    Observed band size: 97, 70 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1 and 2: 3 minutes; Lane 3: 10 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).

  • Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat liver lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : Mouse heart lysate
    Lane 5 : Mouse kidney lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 7 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 9 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 97, 70 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lanes 3 and 4: 136 seconds; Lane 4: 3 minutes; Lane 5: 15 seconds; Lanes 6,7,8 and 9: 103 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).

  • Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Lanes 1-4 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/5000 dilution
    Lanes 5-6 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 5 : 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate
    Lane 6 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 97, 70 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1 and 2: 37 seconds; Lanes 3 and 4: 15 seconds; Lanes 5 and 6: 81 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).

  • Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab217572? Please let us know so that we can cite the reference in this datasheet.

ab217572 has been referenced in 1 publication.

  • Omoteyama K  et al. Identification of novel substrates of a disintegrin and metalloprotease 17 by specific labeling of surface proteins. Heliyon 6:e05804 (2020). PubMed: 33385093

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