Recombinant
RabMAb

Recombinant Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Overview

  • Product name

    Anti-LGALS3BP antibody [EPR21757-33]
    See all LGALS3BP primary antibodies
  • Description

    Rabbit monoclonal [EPR21757-33] to LGALS3BP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human LGALS3BP aa 1-400. The exact sequence is proprietary.
    Database link: Q08380

  • Positive control

    • WB: Human fetal brain, fetal kidney and heart lysate; HepG2, C6, RAW 264.7, PC-12, NIH/3T3, MCF7, A549, HeLa, 4T1 and Hepa1-6 whole cell lysate; Rat brain, liver and spleen lysate. Mouse heart and kidney lysate. Flow: MCF7 and HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21757-33
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 65 kDa.
Flow Cyt 1/60.

Target

  • Function

    Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
  • Tissue specificity

    Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
  • Sequence similarities

    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 1 SRCR domain.
  • Cellular localization

    Secreted. Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 90K antibody
    • 90K MAC 2 BP antibody
    • Basement membrane autoantigen p105 antibody
    • BTBD17B antibody
    • Galectin 3 binding protein antibody
    • Galectin-3-binding protein antibody
    • L3 antigen antibody
    • Lectin galactoside binding soluble 3 binding protein antibody
    • Lectin galactoside-binding soluble 3-binding protein antibody
    • LG3BP_HUMAN antibody
    • LGALS3BP antibody
    • M2BP antibody
    • Mac 2 binding protein antibody
    • Mac 2 BP antibody
    • Mac-2 BP antibody
    • Mac-2-binding protein antibody
    • Mac2 BP antibody
    • MAC2BP antibody
    • Serum protein 90K antibody
    • TANGO10B antibody
    • Transport and golgi organization 10 homolog B antibody
    • Tumor associated antigen 90K antibody
    • Tumor-associated antigen 90K antibody
    see all

Images

  • All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

    Predicted band size: 65 kDa
    Observed band size: 97, 70 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1 and 2: 3 minutes; Lane 3: 10 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat liver lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : Mouse heart lysate
    Lane 5 : Mouse kidney lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 7 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 9 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 97, 70 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lanes 3 and 4: 136 seconds; Lane 4: 3 minutes; Lane 5: 15 seconds; Lanes 6,7,8 and 9: 103 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).

  • Lanes 1-4 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/5000 dilution
    Lanes 5-6 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 5 : 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate
    Lane 6 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 97, 70 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1 and 2: 37 seconds; Lanes 3 and 4: 15 seconds; Lanes 5 and 6: 81 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

References

ab217572 has not yet been referenced specifically in any publications.

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