Recombinant Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21757-33] to LGALS3BP
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-LGALS3BP antibody [EPR21757-33]
See all LGALS3BP primary antibodies -
Description
Rabbit monoclonal [EPR21757-33] to LGALS3BP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human LGALS3BP aa 1-400. The exact sequence is proprietary.
Database link: Q08380 -
Positive control
- WB: Human fetal brain, fetal kidney and heart lysate; HepG2, C6, RAW 264.7, PC-12, NIH/3T3, MCF7, A549, HeLa, 4T1 and Hepa1-6 whole cell lysate; Rat brain, liver and spleen lysate. Mouse heart and kidney lysate. Flow: MCF7 and HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21757-33 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/60.
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WB |
1/1000. Predicted molecular weight: 65 kDa.
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Notes |
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Flow Cyt (Intra)
1/60. |
WB
1/1000. Predicted molecular weight: 65 kDa. |
Target
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Function
Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. -
Tissue specificity
Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. -
Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 1 SRCR domain. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 3959 Human
- Entrez Gene: 19039 Mouse
- Entrez Gene: 245955 Rat
- Omim: 600626 Human
- SwissProt: Q08380 Human
- SwissProt: Q07797 Mouse
- SwissProt: O70513 Rat
- Unigene: 514535 Human
see all -
Alternative names
- 90K antibody
- 90K MAC 2 BP antibody
- Basement membrane autoantigen p105 antibody
see all
Images
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All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution
Predicted band size: 65 kDa
Observed band size: 97, 70 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1 and 2: 3 minutes; Lane 3: 10 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
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All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Rat spleen lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse kidney lysate
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 7 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 9 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 97, 70 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 15 seconds; Lanes 3 and 4: 136 seconds; Lane 4: 3 minutes; Lane 5: 15 seconds; Lanes 6,7,8 and 9: 103 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).
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Lanes 1-4 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/5000 dilution
Lanes 5-6 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate
Lane 6 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 97, 70 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1 and 2: 37 seconds; Lanes 3 and 4: 15 seconds; Lanes 5 and 6: 81 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab217572 has been referenced in 1 publication.
- Omoteyama K et al. Identification of novel substrates of a disintegrin and metalloprotease 17 by specific labeling of surface proteins. Heliyon 6:e05804 (2020). PubMed: 33385093