RecombinantRabMAb

Recombinant Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)

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Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)
  • Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)
  • Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21757-33] to LGALS3BP - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free
    See all LGALS3BP primary antibodies

  • Description

    Rabbit monoclonal [EPR21757-33] to LGALS3BP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human LGALS3BP aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q08380

  • Positive control

    • Flow Cyt: HepG2 cells.

  • General notes

    Ab237031 is the carrier-free version of ab217572. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab237031 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21757-33

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.

  • Tissue specificity

    Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.

  • Sequence similarities

    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 1 SRCR domain.

  • Cellular localization

    Secreted. Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 90K antibody
    • 90K MAC 2 BP antibody
    • Basement membrane autoantigen p105 antibody
    • BTBD17B antibody
    • Galectin 3 binding protein antibody
    • Galectin-3-binding protein antibody
    • L3 antigen antibody
    • Lectin galactoside binding soluble 3 binding protein antibody
    • Lectin galactoside-binding soluble 3-binding protein antibody
    • LG3BP_HUMAN antibody
    • LGALS3BP antibody
    • M2BP antibody
    • Mac 2 binding protein antibody
    • Mac 2 BP antibody
    • Mac-2 BP antibody
    • Mac-2-binding protein antibody
    • Mac2 BP antibody
    • MAC2BP antibody
    • Serum protein 90K antibody
    • TANGO10B antibody
    • Transport and golgi organization 10 homolog B antibody
    • Tumor associated antigen 90K antibody
    • Tumor-associated antigen 90K antibody
    see all

Images

  • Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)
    Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217572).

  • Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)
    Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217572).

  • Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)
    Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (ab237031)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab237031? Please let us know so that we can cite the reference in this datasheet.

ab237031 has not yet been referenced specifically in any publications.

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