Key features and details
- Mouse monoclonal [OTI3D6] to LGALS3BP
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-LGALS3BP antibody [OTI3D6]
See all LGALS3BP primary antibodies
DescriptionMouse monoclonal [OTI3D6] to LGALS3BP
Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Recombinant fragment corresponding to Human LGALS3BP aa 19-300. (NP_005558) produced in E.coli.
VNDGDMRLADGGATNQGRVEIFYRGQWGTVCDNLWDLTDASVVCRALGFE NATQALGRAAFGQGSGPIMLDEVQCTGTEASLADCKSLGWLKSNCRHERD AGVVCTNETRSTHTLDLSRELSEALGQIFDSQRGCDLSISVNVQGEDALG FCGHTVILTANLEAQALWKEPGSNVTMSVDAECVPMVRDLLRYFYSRRID ITLSSVKCFHKLASAYGARQLQGYCASLFAILLPQDPSFQMPLDLYAYAV ATGDALLEKLCLQFLAWNFEALTQAEAWPSVP
Database link: Q08380
- WB: HEK-293T transfected with r pCMV6-ENTRY LGALS3BP cDNA, whole cell lysate. IHC-P: Human lung carcinoma, endometrium adenocarcinoma, prostate and bladder carcinoma tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY LGALS3BP cDNA. Flow: HEK-293T cells.
Clone OTI3D6 (formerly 3D6).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Heat induced epitope retrieval by 10mM citric buffer, pH 6.0, 100°C for 10 minutes.
|WB||1/500 - 1/2000. Predicted molecular weight: 65 kDa.|
FunctionPromotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
Tissue specificityUbiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
Sequence similaritiesContains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 1 SRCR domain.
Cellular localizationSecreted. Secreted > extracellular space > extracellular matrix.
- Information by UniProt
- 90K antibody
- 90K MAC 2 BP antibody
- Basement membrane autoantigen p105 antibody
All lanes : Anti-LGALS3BP antibody [OTI3D6] (ab236509) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cDNA, whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY LGALS3BP cDNA, whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 65 kDa
Paraffin-embedded human lung carcinoma tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium adenocarcinoma tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human bladder carcinoma tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.
COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with pCMV6-ENTRY LGALS3BP cDNA stained for LGALS3BP (green) using ab236509 at 1/100 dilution in ICC/IF.
Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with empty vector control (blue) or vector expressing LGALS3BP (red), labeling LGALS3BP with ab236509 at 1/100 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236509 has not yet been referenced specifically in any publications.