Overview

  • Product name
  • Description
    Rabbit polyclonal to Lgi1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Lgi1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab33114.)

  • Positive control
    • WB: Rat and mouse brain tissue lysate. ICC/IF: CHO cells. IHC-FoFr: Rat spinal cord sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab30868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
  • Tissue specificity
    Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
  • Involvement in disease
    Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
  • Sequence similarities
    Contains 7 EAR repeats.
    Contains 3 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Secreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADLTE antibody
    • ADPAEF antibody
    • ADPEAF antibody
    • Epitempin 1 antibody
    • EPITEMPIN antibody
    • Epitempin-1 antibody
    • EPT antibody
    • ETL1 antibody
    • IB1099 antibody
    • leucine rich glioma inactivated 1 antibody
    • Leucine rich glioma-inactivated protein 1 antibody
    • Leucine-rich glioma-inactivated protein 1 antibody
    • LGI1 antibody
    • LGI1_HUMAN antibody
    • OTTHUMP00000020121 antibody
    • OTTHUMP00000020122 antibody
    see all

Images

  • All lanes : Anti-Lgi1 antibody (ab30868) at 1 µg/ml

    Lane 1 : Mouse brain whole tissue lysate
    Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 3 : Rat brain whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IR Dye 680 Conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • IHC-FoFr image of Lgi1 staining in rat spinal cord sections. The sections used came from animals perfused fixed with 4% paraformaldehyde in 0.2M phosphate buffer. Following postfixation in the same fixative overnight, the tissues were cryoprotected in 30% sucrose overnight. Tissues were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique. Image B is a higher power magnification of the Image A, at the level of the interface between the white and grey matter in the lamina V. Numbers in image A are Rexed's laminae numbers.

    See Abreview

  • ab30868 at 10 µg/ml staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized and blocked before incubation with the antibody for 16 hours. An Alexa Fluor ® 488 conjugated goat polyclonal antibody was used as the secondary.

    See Abreview

  • Anti-Lgi1 antibody (ab30868) at 1/1000 dilution + rat brain tissue lysate at 30 µg

    Secondary
    HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa


    Exposure time: 5 minutes

    See Abreview

References

This product has been referenced in:
  • Yamagata A  et al. Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22. Nat Commun 9:1546 (2018). Read more (PubMed: 29670100) »
  • Dazzo E  et al. LGI1 tumor tissue expression and serum autoantibodies in patients with primary malignant glioma. Clin Neurol Neurosurg 170:27-33 (2018). Read more (PubMed: 29723732) »
See all 20 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Rat Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde

Dr. Sophie Pezet

Verified customer

Submitted Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
25 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3%

Abcam user community

Verified customer

Submitted Jan 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO)
Specification
CHO
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.08 % to 0.2 % triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 24 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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