Anti-Lgi1/EPT antibody (ab228613)
Key features and details
- Rabbit polyclonal to Lgi1/EPT
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Lgi1/EPT antibody
See all Lgi1/EPT primary antibodies -
Description
Rabbit polyclonal to Lgi1/EPT -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Chimpanzee, Rhesus monkey -
Immunogen
Recombinant fragment within Human Lgi1/EPT (internal sequence). The exact sequence is proprietary.
Database link: O95970 -
Positive control
- WB: A431 whole cell lysate. ICC/IF: DIV10 rat E18 primary hippocampal neurons.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 64 kDa. |
Target
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Function
Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival. -
Tissue specificity
Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level). -
Involvement in disease
Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs. -
Sequence similarities
Contains 7 EAR repeats.
Contains 3 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 450619 Chimpanzee
- Entrez Gene: 617080 Cow
- Entrez Gene: 9211 Human
- Entrez Gene: 56839 Mouse
- Entrez Gene: 252892 Rat
- Entrez Gene: 700520 Rhesus monkey
- Omim: 604619 Human
- SwissProt: Q1EGL2 Chimpanzee
see all -
Alternative names
- ADLTE antibody
- ADPAEF antibody
- ADPEAF antibody
see all
Images
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Anti-Lgi1/EPT antibody (ab228613) at 1/5000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 64 kDa7.5% SDS-PAGE gel.
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4% paraformaldehyde-fixed DIV10 rat E18 primary hippocampal neurons stained for Lgi1/EPT (green) using ab228613 at 1/500 dilution in ICC/IF.
Nuclear counter stain: DAPI (blue). beta Tubulin 3 was stained with an anti-beta Tubulin 3 antibody at 1/500 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228613 has not yet been referenced specifically in any publications.