Key features and details
- Rabbit polyclonal to Lgi1/EPT
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Lgi1/EPT antibody
See all Lgi1/EPT primary antibodies
DescriptionRabbit polyclonal to Lgi1/EPT
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Chimpanzee, Rhesus monkey
Recombinant fragment within Human Lgi1/EPT (internal sequence). The exact sequence is proprietary.
Database link: O95970
- WB: A431 whole cell lysate. ICC/IF: DIV10 rat E18 primary hippocampal neurons.
This product was previously labelled as Lgi1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 64 kDa.|
FunctionRegulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
Tissue specificityPredominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
Involvement in diseaseDefects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
Sequence similaritiesContains 7 EAR repeats.
Contains 3 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Cellular localizationSecreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum.
- Information by UniProt
- ADLTE antibody
- ADPAEF antibody
- ADPEAF antibody
Anti-Lgi1/EPT antibody (ab228613) at 1/5000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 64 kDa
7.5% SDS-PAGE gel.
4% paraformaldehyde-fixed DIV10 rat E18 primary hippocampal neurons stained for Lgi1/EPT (green) using ab228613 at 1/500 dilution in ICC/IF.
Nuclear counter stain: DAPI (blue). beta Tubulin 3 was stained with an anti-beta Tubulin 3 antibody at 1/500 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228613 has not yet been referenced specifically in any publications.