Overview

  • Product name

    Anti-Lgi1/EPT antibody [EPR9084]
    See all Lgi1/EPT primary antibodies
  • Description

    Rabbit monoclonal [EPR9084] to Lgi1/EPT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Lgi1/EPT aa 200-300.
    Database link: O95970

  • Positive control

    • HeLa and U87-MG lysates; Human brain tissue.
  • General notes

     

     

     This product was previously labelled as Lgi1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
    • Tissue specificity

      Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
    • Involvement in disease

      Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
    • Sequence similarities

      Contains 7 EAR repeats.
      Contains 3 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
    • Post-translational
      modifications

      Glycosylated.
    • Cellular localization

      Secreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADLTE antibody
      • ADPAEF antibody
      • ADPEAF antibody
      • Epitempin 1 antibody
      • EPITEMPIN antibody
      • Epitempin-1 antibody
      • EPT antibody
      • ETL1 antibody
      • IB1099 antibody
      • leucine rich glioma inactivated 1 antibody
      • Leucine rich glioma-inactivated protein 1 antibody
      • Leucine-rich glioma-inactivated protein 1 antibody
      • LGI1 antibody
      • LGI1_HUMAN antibody
      • OTTHUMP00000020121 antibody
      • OTTHUMP00000020122 antibody
      see all

    Images

    • All lanes : Anti-Lgi1/EPT antibody [EPR9084] (ab137045) at 1/1000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : HeLa lysate
      Lane 3 : U87-MG lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 64 kDa

    • Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Lgi1/EPT with ab137045 at 1/100 dilution.

    References

    ab137045 has not yet been referenced specifically in any publications.

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