Key features and details
- Mouse monoclonal [N283/7] to Lgi1/EPT
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Product nameAnti-Lgi1/EPT antibody [N283/7]
See all Lgi1/EPT primary antibodies
DescriptionMouse monoclonal [N283/7] to Lgi1/EPT
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human, Chimpanzee, Orangutan
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S283-7 to N283/7, both clone numbers name the same antibody clone.
This product was previously labelled as Lgi1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 64 kDa).|
FunctionRegulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
Tissue specificityPredominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
Involvement in diseaseDefects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
Sequence similaritiesContains 7 EAR repeats.
Contains 3 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Cellular localizationSecreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum.
- Information by UniProt
- ADLTE antibody
- ADPAEF antibody
- ADPEAF antibody
SK-N-BE (Human neuroblastoma cell line ) cells stained for Lgi1/EPT (Green) using ab186296 at a 1/100 dilution (1 hour at RT) in ICC/IF. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.
Cells were fixed with 4% formaldehyde for 15 minutes at RT.
Anti-Lgi1/EPT antibody [N283/7] (ab186296) at 1/1000 dilution (for 1 hour at RT) + Rat brain lysate, for 1 hour at RT at 10 µg
Goat Anti-Mouse HRP at 1/200 dilution
Predicted band size: 64 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab186296 has not yet been referenced specifically in any publications.