Overview

  • Product name

    Anti-Lgi1/EPT antibody [N283/7] (FITC)
    See all Lgi1/EPT primary antibodies
  • Description

    Mouse monoclonal [N283/7] to Lgi1/EPT (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein (His-tag) corresponding to Mouse Lgi1/EPT aa 37-113 (N terminal). LRRNT domain and first LRR repeat.
    Database link: Q9JIA1

  • Positive control

    • Rat brain tissue lysate
  • General notes

    The clone number has been updated from S283-7 to N283/7, both clone numbers name the same antibody clone.

     

     This product was previously labelled as Lgi1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab186274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 64 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
  • Tissue specificity

    Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
  • Involvement in disease

    Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
  • Sequence similarities

    Contains 7 EAR repeats.
    Contains 3 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Secreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADLTE antibody
    • ADPAEF antibody
    • ADPEAF antibody
    • Epitempin 1 antibody
    • EPITEMPIN antibody
    • Epitempin-1 antibody
    • EPT antibody
    • ETL1 antibody
    • IB1099 antibody
    • leucine rich glioma inactivated 1 antibody
    • Leucine rich glioma-inactivated protein 1 antibody
    • Leucine-rich glioma-inactivated protein 1 antibody
    • LGI1 antibody
    • LGI1_HUMAN antibody
    • OTTHUMP00000020121 antibody
    • OTTHUMP00000020122 antibody
    see all

References

ab186274 has not yet been referenced specifically in any publications.

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