Overview

  • Product name

  • Description

    Rabbit polyclonal to LGN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human LGN aa 627-677. (AAH27732.1)

  • Positive control

    • HeLa or 293T whole cell lysate
  • General notes

    Protein previously labeled as GPSM2.

Properties

Applications

Our Abpromise guarantee covers the use of ab109594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Defects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
  • Sequence similarities

    Belongs to the GPSM family.
    Contains 4 GoLoco domains.
    Contains 8 TPR repeats.
  • Cellular localization

    Cytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • DFNB82 antibody
    • G protein signalling modulator 2 (AGS3 like C. elegans) antibody
    • G protein signalling modulator 2 antibody
    • G-protein-signaling modulator 2 antibody
    • Gpsm2 antibody
    • GPSM2_HUMAN antibody
    • HGNC:29501 antibody
    • LGN antibody
    • LGN protein antibody
    • Mosaic protein LGN antibody
    • Pins antibody
    see all

Images

  • All lanes : Anti-LGN antibody (ab109594) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 77 kDa


    Exposure time: 3 minutes
  • Detection of LGN by Western Blot of Immunprecipitate.
    ab109594 at 1µg/ml staining LGN in HeLa whole cell lysate immunoprecipitated using ab109594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:

  • Freeman S  et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). Read more (PubMed: 31321879) »
See 1 Publication for this product

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