Key features and details
- Rabbit polyclonal to LGN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LGN antibody
See all LGN primary antibodies
DescriptionRabbit polyclonal to LGN
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to Human LGN aa 627-677. (AAH27732.1)
- HeLa or 293T whole cell lysate
Protein previously labeled as GPSM2.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab109594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 77 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPlays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
Sequence similaritiesBelongs to the GPSM family.
Contains 4 GoLoco domains.
Contains 8 TPR repeats.
Cellular localizationCytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
- Information by UniProt
- DFNB82 antibody
- G protein signalling modulator 2 (AGS3 like C. elegans) antibody
- G protein signalling modulator 2 antibody
All lanes : Anti-LGN antibody (ab109594) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 3 minutes
Detection of LGN by Western Blot of Immunprecipitate.
ab109594 at 1µg/ml staining LGN in HeLa whole cell lysate immunoprecipitated using ab109594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
ab109594 has been referenced in 1 publication.
- Freeman S et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). PubMed: 31321879