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  1. Link

    lgn-antibody-ab109594.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Anti-LGN antibody (ab109594)

  • Datasheet
  • SDS
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Western blot - Anti-LGN antibody (ab109594)
  • Immunoprecipitation - Anti-LGN antibody (ab109594)

Key features and details

  • Rabbit polyclonal to LGN
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Knockout
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Human GPSM2 (LGN) knockout HeLa cell line (ab264715)

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Overview

  • Product name

    Anti-LGN antibody
    See all LGN primary antibodies
  • Description

    Rabbit polyclonal to LGN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human LGN aa 627-677. (AAH27732.1)

  • Positive control

    • HeLa or 293T whole cell lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • Regulators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP
Use at 2-5 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP
Use at 2-5 µg/mg of lysate.

Target

  • Function

    Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Defects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
  • Sequence similarities

    Belongs to the GPSM family.
    Contains 4 GoLoco domains.
    Contains 8 TPR repeats.
  • Cellular localization

    Cytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
  • Target information above from: UniProt accession P81274 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29899 Human
    • Omim: 609245 Human
    • SwissProt: P81274 Human
    • Unigene: 584901 Human
    • Unigene: 658489 Human
    • Alternative names

      • DFNB82 antibody
      • G protein signalling modulator 2 (AGS3 like C. elegans) antibody
      • G protein signalling modulator 2 antibody
      • G-protein-signaling modulator 2 antibody
      • Gpsm2 antibody
      • GPSM2_HUMAN antibody
      • HGNC:29501 antibody
      • LGN antibody
      • LGN protein antibody
      • Mosaic protein LGN antibody
      • Pins antibody
      see all

    Images

    • Western blot - Anti-LGN antibody (ab109594)
      Western blot - Anti-LGN antibody (ab109594)
      All lanes : Anti-LGN antibody (ab109594) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 77 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-LGN antibody (ab109594)
      Immunoprecipitation - Anti-LGN antibody (ab109594)

      Detection of LGN by Western Blot of Immunprecipitate.
      ab109594 at 1µg/ml staining LGN in HeLa whole cell lysate immunoprecipitated using ab109594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab109594? Please let us know so that we can cite the reference in this datasheet.

    ab109594 has been referenced in 1 publication.

    • Freeman S  et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). PubMed: 31321879

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