Anti-LGN antibody (ab109594)

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Key features and details

  • Rabbit polyclonal to LGN
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-LGN antibody
    See all LGN primary antibodies

  • Description

    Rabbit polyclonal to LGN

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla

  • Immunogen

    Synthetic peptide corresponding to Human LGN aa 627-677. (AAH27732.1)

  • Positive control

    • HeLa or 293T whole cell lysate

  • General notes

    Protein previously labeled as GPSM2.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

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Properties

Applications

Our Abpromise guarantee covers the use of ab109594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.

  • Tissue specificity

    Ubiquitously expressed.

  • Involvement in disease

    Defects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.

  • Sequence similarities

    Belongs to the GPSM family.
    Contains 4 GoLoco domains.
    Contains 8 TPR repeats.

  • Cellular localization

    Cytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
  • Information by UniProt

  • Database links

    • Alternative names

      • DFNB82 antibody
      • G protein signalling modulator 2 (AGS3 like C. elegans) antibody
      • G protein signalling modulator 2 antibody
      • G-protein-signaling modulator 2 antibody
      • Gpsm2 antibody
      • GPSM2_HUMAN antibody
      • HGNC:29501 antibody
      • LGN antibody
      • LGN protein antibody
      • Mosaic protein LGN antibody
      • Pins antibody
      see all

    Images

    • Western blot - Anti-LGN antibody (ab109594)
      Western blot - Anti-LGN antibody (ab109594)
      All lanes : Anti-LGN antibody (ab109594) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 77 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-LGN antibody (ab109594)
      Immunoprecipitation - Anti-LGN antibody (ab109594)

      Detection of LGN by Western Blot of Immunprecipitate.
      ab109594 at 1µg/ml staining LGN in HeLa whole cell lysate immunoprecipitated using ab109594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

    References (1)

    Publishing research using ab109594? Please let us know so that we can cite the reference in this datasheet.

    ab109594 has been referenced in 1 publication.

    • Freeman S  et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). PubMed: 31321879

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