Anti-LGN antibody (ab109594)
Key features and details
- Rabbit polyclonal to LGN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LGN antibody
See all LGN primary antibodies -
Description
Rabbit polyclonal to LGN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human LGN aa 627-677. (AAH27732.1)
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Positive control
- HeLa or 293T whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction. -
Sequence similarities
Belongs to the GPSM family.
Contains 4 GoLoco domains.
Contains 8 TPR repeats. -
Cellular localization
Cytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase. - Information by UniProt
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Database links
- Entrez Gene: 29899 Human
- Omim: 609245 Human
- SwissProt: P81274 Human
- Unigene: 584901 Human
- Unigene: 658489 Human
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Alternative names
- DFNB82 antibody
- G protein signalling modulator 2 (AGS3 like C. elegans) antibody
- G protein signalling modulator 2 antibody
see all
Images
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Western blot - Anti-LGN antibody (ab109594)All lanes : Anti-LGN antibody (ab109594) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-LGN antibody (ab109594)Detection of LGN by Western Blot of Immunprecipitate.
ab109594 at 1µg/ml staining LGN in HeLa whole cell lysate immunoprecipitated using ab109594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab109594 has been referenced in 1 publication.
- Freeman S et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). PubMed: 31321879