- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-LGN antibody
See all LGN primary antibodies -
DescriptionRabbit polyclonal to LGN
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Host speciesRabbit
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Tested applicationsSuitable for: IPmore details
Unsuitable for: WB -
Species reactivityReacts with: Human
Predicted to work with: Cow, Chimpanzee, Orangutan
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Immunogen
Synthetic peptide corresponding to Human LGN aa 450-500. (AAH27732.1)
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Positive control
- HeLa whole cell lysate
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General notes
Protein previously labeled as GPSM2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab110147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. |
Target
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FunctionPlays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
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Tissue specificityUbiquitously expressed.
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Involvement in diseaseDefects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
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Sequence similaritiesBelongs to the GPSM family.
Contains 4 GoLoco domains.
Contains 8 TPR repeats. -
Cellular localizationCytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
- Information by UniProt
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Database links
- Entrez Gene: 29899 Human
- Omim: 609245 Human
- SwissProt: P81274 Human
- Unigene: 584901 Human
- Unigene: 658489 Human
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Alternative names
- DFNB82 antibody
- G protein signalling modulator 2 (AGS3 like C. elegans) antibody
- G protein signalling modulator 2 antibody
see all
Images
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Immunoprecipitation - Anti-LGN antibody (ab110147)Detection of LGN in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab110147 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-GPSM2 antibody which recognizes a downstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
References
ab110147 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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