Anti-LGN antibody (ab110147)
Key features and details
- Rabbit polyclonal to LGN
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LGN antibody
See all LGN primary antibodies -
Description
Rabbit polyclonal to LGN -
Host species
Rabbit -
Tested Applications & Species
Application Species IP Human -
Immunogen
Synthetic peptide corresponding to Human LGN aa 450-500. (AAH27732.1)
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Positive control
- HeLa whole cell lysate
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General notes
Protein previously labeled as GPSM2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IP |
Human
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All applications |
Cow
Chimpanzee
Orangutan
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Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction. -
Sequence similarities
Belongs to the GPSM family.
Contains 4 GoLoco domains.
Contains 8 TPR repeats. -
Cellular localization
Cytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase. - Information by UniProt
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Database links
- Entrez Gene: 29899 Human
- Omim: 609245 Human
- SwissProt: P81274 Human
- Unigene: 584901 Human
- Unigene: 658489 Human
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Alternative names
- DFNB82 antibody
- G protein signalling modulator 2 (AGS3 like C. elegans) antibody
- G protein signalling modulator 2 antibody
see all
Images
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Detection of LGN in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab110147 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-LGN antibody which recognizes a downstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110147 has not yet been referenced specifically in any publications.