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    lgn-antibody-ab110147.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Anti-LGN antibody (ab110147)

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Immunoprecipitation - Anti-LGN antibody (ab110147)

    Key features and details

    • Rabbit polyclonal to LGN
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-LGN antibody
      See all LGN primary antibodies
    • Description

      Rabbit polyclonal to LGN
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      IP
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human LGN aa 450-500. (AAH27732.1)

    • Positive control

      • HeLa whole cell lysate
    • General notes

      Protein previously labeled as GPSM2.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Spindle
      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • Heterotrimeric G Proteins
      • Regulators

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab29545)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab110147 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IP
    Human
    All applications
    Cow
    Chimpanzee
    Orangutan
    Application Abreviews Notes
    IP
    Use at 2-5 µg/mg of lysate.
    Notes
    IP
    Use at 2-5 µg/mg of lysate.

    Target

    • Function

      Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
    • Tissue specificity

      Ubiquitously expressed.
    • Involvement in disease

      Defects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
    • Sequence similarities

      Belongs to the GPSM family.
      Contains 4 GoLoco domains.
      Contains 8 TPR repeats.
    • Cellular localization

      Cytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
    • Target information above from: UniProt accession P81274 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 29899 Human
      • Omim: 609245 Human
      • SwissProt: P81274 Human
      • Unigene: 584901 Human
      • Unigene: 658489 Human
      • Alternative names

        • DFNB82 antibody
        • G protein signalling modulator 2 (AGS3 like C. elegans) antibody
        • G protein signalling modulator 2 antibody
        • G-protein-signaling modulator 2 antibody
        • Gpsm2 antibody
        • GPSM2_HUMAN antibody
        • HGNC:29501 antibody
        • LGN antibody
        • LGN protein antibody
        • Mosaic protein LGN antibody
        • Pins antibody
        see all

      Images

      • Immunoprecipitation - Anti-LGN antibody (ab110147)
        Immunoprecipitation - Anti-LGN antibody (ab110147)

        Detection of LGN in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab110147 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-LGN antibody which recognizes a downstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

      Protocols

      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab110147? Please let us know so that we can cite the reference in this datasheet.

      ab110147 has not yet been referenced specifically in any publications.

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