Key features and details
- Rabbit polyclonal to LGN
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LGN antibody
See all LGN primary antibodies
DescriptionRabbit polyclonal to LGN
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: SK-BR-3 cell lysate. IHC-P: Human cerebellum tissue. ICC/IF: U-251 MG cells.
Protein previously labeled as GPSM2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/250. Predicted molecular weight: 77 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionPlays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
Sequence similaritiesBelongs to the GPSM family.
Contains 4 GoLoco domains.
Contains 8 TPR repeats.
Cellular localizationCytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
- Information by UniProt
- DFNB82 antibody
- G protein signalling modulator 2 (AGS3 like C. elegans) antibody
- G protein signalling modulator 2 antibody
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for LGN (green) using ab224238 at 4 µg/ml in ICC/IF.
Paraffin-embedded human cerebellum tissue stained for LGN using ab224238 at 1/500 dilution in immunohistochemical analysis.
Anti-LGN antibody (ab224238) at 1/100 dilution + SK-BR-3 (human mammary gland adenocarcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 77 kDa
ab224238 has not yet been referenced specifically in any publications.