Key features and details
- Rabbit polyclonal to LGR5
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-LGR5 antibody
See all LGR5 primary antibodies
DescriptionRabbit polyclonal to LGR5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human LGR5.
Database link: O75473
- WB: HeLa and HepG2 extracts. Mouse skeletal muscle extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab237598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 99 kDa.|
FunctionOrphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
Tissue specificityExpressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.
Cellular localizationCell membrane.
- Information by UniProt
- FEX antibody
- G protein coupled receptor 49 antibody
- G protein coupled receptor 67 antibody
All lanes : Anti-LGR5 antibody (ab237598) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts
Lane 3 : Mouse skeletal muscle extracts
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 99 kDa
Blocking buffer: 3% non-fat dry milk in TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237598 has not yet been referenced specifically in any publications.