Overview

  • Product name
    Anti-LGR5 antibody [EPR3065Y]
    See all LGR5 primary antibodies
  • Description
    Rabbit monoclonal [EPR3065Y] to LGR5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LGR5 aa 800-900 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab221221)

  • Positive control
    • Fetal skeletal muscle lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 100 kDa.Can be blocked with LGR5 peptide (ab221221).
IP 1/20.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
    • Tissue specificity
      Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
      Contains 16 LRR (leucine-rich) repeats.
      Contains 1 LRRNT domain.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • FEX antibody
      • G protein coupled receptor 49 antibody
      • G protein coupled receptor 67 antibody
      • g protein-coupled receptor fex antibody
      • G-protein coupled receptor 49 antibody
      • G-protein coupled receptor 67 antibody
      • G-protein coupled receptor HG38 antibody
      • GPCR GPR49 antibody
      • GPR 49 antibody
      • GPR 67 antibody
      • GPR49 antibody
      • GPR67 antibody
      • GRP 49 antibody
      • GRP49 antibody
      • HG 38 antibody
      • HG38 antibody
      • Leucine rich repeat containing G protein coupled receptor 5 antibody
      • Leucine-rich repeat-containing G-protein coupled receptor 5 antibody
      • LGR 5 antibody
      • LGR5 antibody
      • LGR5_HUMAN antibody
      • MGC117008 antibody
      • Orphan G protein coupled receptor HG38 antibody
      see all

    Images

    • Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution + Fetal
      skeletal muscle lysate at 10 µg

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 100 kDa
      Observed band size: 100 kDa

    References

    This product has been referenced in:
    • Ullmann P  et al. Tumor suppressor miR-215 counteracts hypoxia-induced colon cancer stem cell activity. Cancer Lett 450:32-41 (2019). Read more (PubMed: 30790680) »
    • Yang B  et al. ß-catenin, leucine-rich repeat-containing G protein-coupled receptor 5 and GATA-binding factor 6 are associated with the normal mucosa-adenoma-adenocarcinoma sequence of colorectal tumorigenesis. Oncol Lett 15:2287-2295 (2018). Read more (PubMed: 29434936) »
    See all 26 Publications for this product

    Customer reviews and Q&As

    1-10 of 24 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (skin)
    Permeabilization
    Yes - citrate
    Specification
    skin
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted May 30 2018

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    Yes - BSA
    Specification
    Colon
    Blocking step
    Hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde

    Vinicius Kannen Cardoso

    Verified customer

    Submitted Sep 09 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skin)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde

    Ahmar Aziz

    Verified customer

    Submitted Dec 24 2015

    This product is known to not work in this application or species.
    Application
    Flow Cytometry
    Fixation
    none
    Permeabilization
    No
    Sample
    Mouse Cell (colon)
    Specification
    colon
    Gating Strategy
    High FSC/SSC log scale
    Preparation
    Cell harvesting/tissue preparation method: isolate colon, collagenase digestion
    Sample buffer: DMEM

    Abcam user community

    Verified customer

    Submitted Aug 20 2013

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SW620 Colon)
    Gel Running Conditions
    Reduced Denaturing (7)
    Loading amount
    100 µg
    Treatment
    siRNA - knockdown
    Specification
    SW620 Colon
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Apr 08 2013

    Answer


    Protein G beads were indeed used with ab75850 following this protocol for IP.

    Read More

    Question
    Answer


    I have contacted the lab and ab75850 tested positive in IP (image attached). Rat heart is the only sample we have tested. Our recommended IP protocol is described at the following link: http://www.epitomics.com/products/support/ip, and is the same protocol we used when testing ab75850.

    Read More
    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
    Permeabilization
    No
    Specification
    Intestine
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 12 2013

    Question
    Answer

    Thank you for your enquiry.

    I can confirm that ab75850 Anti-GPCR GPR49 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

    I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

    I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question
    Answer

    Thank you for your reply.

    I have gone ahead and processed your request for a free of charge replacement antibody and you should have it by 10.30am tomorrow morning. Your new order number is 1126740.

    If there is anything else I can help you with, please let me know.

    Read More

    1-10 of 24 Abreviews or Q&A

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