Recombinant
RabMAb

Recombinant Anti-LHFPL5 antibody [EPR16285] - BSA and Azide free (ab232650)

Overview

  • Product name

    Anti-LHFPL5 antibody [EPR16285] - BSA and Azide free
    See all LHFPL5 primary antibodies
  • Description

    Rabbit monoclonal [EPR16285] to LHFPL5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LHFPL5 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8TAF8

  • Positive control

    • Flow Cyt: HepG2 cells.
  • General notes

    Ab232650 is the carrier-free version of ab192242. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232650 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16285
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab232650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.

Target

  • Function

    May function in hair bundle morphogenesis.
  • Involvement in disease

    Defects in LHFPL5 are a cause of deafness autosomal recessive type 67 (DFNB67) [MIM:610265]. DFNB67 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities

    Belongs to the LHFP family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deafness, autosomal recessive 67 antibody
    • DFNB67 antibody
    • dJ510O8.8 antibody
    • LHFP-like protein 5 antibody
    • LHFPL5 antibody
    • Lipoma HMGIC fusion partner-like 5 antibody
    • Lipoma HMGIC fusion partner-like 5 protein antibody
    • MGC33835 antibody
    • OTTHUMP00000016278 antibody
    • Tetraspan membrane protein of hair cell stereocilia antibody
    • TMHS antibody
    • TMHS_HUMAN antibody
    see all

Images

  • Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab192242 at 1/60 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192242).

  • Flow cytometric analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells (paraformaldehyde-fixed, 2%) labeling LHFPL5 with ab192242 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192242).

References

ab232650 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232650.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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