RecombinantRabMAb

Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

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Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
  • Flow Cytometry - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
  • Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16285] to LHFPL5 - C-terminal
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LHFPL5 antibody [EPR16285] - C-terminal
    See all LHFPL5 primary antibodies

  • Description

    Rabbit monoclonal [EPR16285] to LHFPL5 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human LHFPL5 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8TAF8

  • Positive control

    • Human fetal brain, human fetal lung, HepG2, mouse brain, mouse heart, mouse kidney, rat kidney and rat heart lysates; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16285

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab192242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP 1/60.
WB 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

Target

  • Function

    May function in hair bundle morphogenesis.

  • Involvement in disease

    Defects in LHFPL5 are a cause of deafness autosomal recessive type 67 (DFNB67) [MIM:610265]. DFNB67 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

  • Sequence similarities

    Belongs to the LHFP family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Deafness, autosomal recessive 67 antibody
    • DFNB67 antibody
    • dJ510O8.8 antibody
    • LHFP-like protein 5 antibody
    • LHFPL5 antibody
    • Lipoma HMGIC fusion partner-like 5 antibody
    • Lipoma HMGIC fusion partner-like 5 protein antibody
    • MGC33835 antibody
    • OTTHUMP00000016278 antibody
    • Tetraspan membrane protein of hair cell stereocilia antibody
    • TMHS antibody
    • TMHS_HUMAN antibody
    see all

Images

  • Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
    Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

    Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab192242 at 1/60 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Flow Cytometry - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
    Flow Cytometry - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

    Flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling LHFPL5 with ab192242 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
    Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
    All lanes : Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
    Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
    Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
    Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
    All lanes : Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 24 kDa

  • Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
    Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

References (1)

Publishing research using ab192242? Please let us know so that we can cite the reference in this datasheet.

ab192242 has been referenced in 1 publication.

  • Li X  et al. Localization of TMC1 and LHFPL5 in auditory hair cells in neonatal and adult mice. FASEB J 33:6838-6851 (2019). PubMed: 30808210

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