Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16285] to LHFPL5 - C-terminal
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LHFPL5 antibody [EPR16285] - C-terminal
See all LHFPL5 primary antibodies -
Description
Rabbit monoclonal [EPR16285] to LHFPL5 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human LHFPL5 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q8TAF8 -
Positive control
- Human fetal brain, human fetal lung, HepG2, mouse brain, mouse heart, mouse kidney, rat kidney and rat heart lysates; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16285 -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab192242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | 1/180. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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IP | 1/60. | |
WB | 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
Target
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Function
May function in hair bundle morphogenesis. -
Involvement in disease
Defects in LHFPL5 are a cause of deafness autosomal recessive type 67 (DFNB67) [MIM:610265]. DFNB67 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Belongs to the LHFP family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 222662 Human
- Entrez Gene: 328789 Mouse
- Entrez Gene: 294303 Rat
- Omim: 609427 Human
- SwissProt: Q8TAF8 Human
- SwissProt: Q4KL25 Mouse
- SwissProt: Q5PPI7 Rat
- Unigene: 367947 Human
see all -
Alternative names
- Deafness, autosomal recessive 67 antibody
- DFNB67 antibody
- dJ510O8.8 antibody
see all
Images
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Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab192242 at 1/60 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
Flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling LHFPL5 with ab192242 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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All lanes : Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 24 kDa -
Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 24 kDa -
All lanes : Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 24 kDa
Protocols
Datasheets and documents
Certificate of Compliance
References (1)
ab192242 has been referenced in 1 publication.
- Li X et al. Localization of TMC1 and LHFPL5 in auditory hair cells in neonatal and adult mice. FASEB J 33:6838-6851 (2019). PubMed: 30808210