Key features and details
- Rabbit polyclonal to LHPP
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-LHPP antibody
DescriptionRabbit polyclonal to LHPP
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- IHC-P: Human kidney, liver and cerebral cortex tissue. WB: Human cerebral cortex tissue lysate; NBT-II cell lysate. ICC/IF: U-2 OS cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityExpressed in liver, kidney and moderately in brain.
Sequence similaritiesBelongs to the HAD-like hydrolase superfamily.
- Information by UniProt
- FLJ44846 antibody
- FLJ46044 antibody
- HDHD2B antibody
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling LHPP using ab254788 at 4µg/ml (green) in ICC/IF.
Paraffin-embedded human kidney tissue stained for LHPP using ab254788 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for LHPP using ab254788 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for LHPP using ab254788 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for LHPP using ab254788 at 1/200 dilution in immunohistochemical analysis.
Anti-LHPP antibody (ab254788) at 0.04 µg/ml + Cerebral cortex tissue lysate
Western blot analysis labeling LHPP with ab254788 at 0.04 ug/ml.
All lanes : Anti-LHPP antibody (ab254788) at 0.4 µg/ml
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NBT-II cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254788 has not yet been referenced specifically in any publications.