Overview

  • Product name

    Anti-Lhx2/LH2 antibody [EPR20449]
    See all Lhx2/LH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20449] to Lhx2/LH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Rat Lhx2/LH2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P36198

  • Positive control

    • WB: His-tagged rat Lhx2/LH2 recombinant protein fragment (aa43-426); E18 rat brain lysate; E12.5 mouse brain lysate. IHC-P: Mouse olfactory epithelium, Nose skin of P0 mouse, Mouse E14.5, Rat olfactory epithelium, Nose skin of P0 rat and rat E14.5 tissues. IHC-Fr: Mouse E14.5 and rat E14.5 tissues. IP: E18 rat brain lysate.
  • General notes

     This product was previously labelled as Lhx2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 44 kDa).
IHC-Fr 1/500.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

IP 1/30.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Domain

    LIM domains are necessary for transcription activation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • hLhx2 antibody
    • Homeobox protein LH-2 antibody
    • Homeobox protein LH2 antibody
    • LH2 antibody
    • LHX2 antibody
    • LHX2_HUMAN antibody
    • LIM homeobox 2 antibody
    • LIM homeobox protein 2 antibody
    • LIM HOX gene 2 antibody
    • LIM/homeobox protein Lhx2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse olfactory epithelium [PMID: 15456728].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on vomeronasal organ of mouse E14.5 [PMID: 27521061].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • All lanes : Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution

    Lane 1 : His-tagged rat Lhx2/LH2 recombinant protein fragment (aa43-426)
    Lane 2 : His-tagged rat Lhx9 recombinant protein fragment (aa54-172)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Lhx2/LH2 was immunoprecipitated from 0.35 mg of E18 rat brain lysate with ab184337 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184337 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: E18 rat brain lysate, 10 μg (Input).

    Lane 2: ab184337 IP in E18 rat brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184337 in E18 rat brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemical analysis of paraffin-embedded Mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of mouse E14.5 [PMID: 25071464].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded rat olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat olfactory epithelium [PMID: 15456728].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on olfactory epithelium of rat E14.5 [PMID: 15456728].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution + E18 rat brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/200 dilution + E12.5 mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Molecular weight observed is consistent with what has been described in the literature, PMID 22028024, PMID 25128627.

  • Immunohistochemical analysis of paraffin-embedded Rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of rat E14.5 [PMID: 25071464].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded nose skin of P0 mouse labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of mouse nose skin [PMID: 20386748].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded nose skin of P0 rat labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of rat nose skin [PMID: 20386748].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

References

This product has been referenced in:

  • Han X  et al. FoxG1 Directly Represses Dentate Granule Cell Fate During Forebrain Development. Front Cell Neurosci 12:452 (2018). Read more (PubMed: 30532694) »
  • Liu B  et al. Forced Expression of Foxg1 in the Cortical Hem Leads to the Transformation of Cajal-Retzius Cells into Dentate Granule Neurons. J Dev Biol 6:N/A (2018). Read more (PubMed: 29949945) »
See all 2 Publications for this product

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