RecombinantRabMAb

Recombinant Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)

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Western blot - Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
  • Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9539] to Lhx2/LH2
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Lhx2/LH2 antibody [EPR9539]
    See all Lhx2/LH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9539] to Lhx2/LH2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Lhx2/LH2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Daudi, HeLa, T.T. and HepG2 cell lysates

  • General notes

     

     

     This product was previously labelled as Lhx2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
      Western blot - Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
      All lanes : Anti-Lhx2/LH2 antibody [EPR9539] (ab140614) at 1/1000 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : T.T. cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 44 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?

    • Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
      Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)

    References (1)

    Publishing research using ab140614? Please let us know so that we can cite the reference in this datasheet.

    ab140614 has been referenced in 1 publication.

    • Liang TS  et al. MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/ß-catenin signaling pathway by down-regulating LHX2. J Exp Clin Cancer Res 38:97 (2019). PubMed: 30791932

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