Recombinant Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
![Western blot - Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)](https://www.abcam.com/ps/products/140/ab140614/Images/Lhx2-Primary-antibodies-ab140614-1.jpg "Western blot - Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9539] to Lhx2/LH2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Lhx2/LH2 antibody [EPR9539]
See all Lhx2/LH2 primary antibodies -
Description
Rabbit monoclonal [EPR9539] to Lhx2/LH2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Lhx2/LH2 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- Daudi, HeLa, T.T. and HepG2 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9539 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
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Function
Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Domain
LIM domains are necessary for transcription activation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9355 Human
- Entrez Gene: 16870 Mouse
- Entrez Gene: 296706 Rat
- Omim: 603759 Human
- SwissProt: P50458 Human
- SwissProt: Q9Z0S2 Mouse
- SwissProt: P36198 Rat
- Unigene: 696425 Human
see all -
Alternative names
- hLhx2 antibody
- Homeobox protein LH-2 antibody
- Homeobox protein LH2 antibody
see all
Images
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Western blot - Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)All lanes : Anti-Lhx2/LH2 antibody [EPR9539] (ab140614) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : T.T. cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
![Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)](https://www.abcam.com/ps/products/140/ab140614/Images/ab140614-2-benefits-of-recombinant-antibodies.png)
Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
Datasheets and documents
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Datasheet download
References (1)
ab140614 has been referenced in 1 publication.
- Liang TS et al. MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/ß-catenin signaling pathway by down-regulating LHX2. J Exp Clin Cancer Res 38:97 (2019). PubMed: 30791932
