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  1. Link

    lhx2lh2-antibody-epr9539-ab140614.pdf

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Neuroscience Cell Type Marker Neural Stem Cell marker
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RecombinantRabMAb

Recombinant Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)

  • Datasheet
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Western blot - Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
  • Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9539] to Lhx2/LH2
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Lhx2/LH2 antibody [EPR9539]
    See all Lhx2/LH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9539] to Lhx2/LH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Lhx2/LH2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Daudi, HeLa, T.T. and HepG2 cell lysates
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9539
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • LIM
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules

Associated products

  • Alternative Versions

    • Anti-Lhx2/LH2 antibody [EPR9539] - BSA and Azide free (ab248904)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 nuclear extract lysate (ab14660)
    • Daudi whole cell lysate (ab3951)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Target

  • Function

    Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Domain

    LIM domains are necessary for transcription activation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P50458 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9355 Human
    • Entrez Gene: 16870 Mouse
    • Entrez Gene: 296706 Rat
    • Omim: 603759 Human
    • SwissProt: P50458 Human
    • SwissProt: Q9Z0S2 Mouse
    • SwissProt: P36198 Rat
    • Unigene: 696425 Human
    • Unigene: 142856 Mouse
    • Unigene: 81063 Rat
    see all
  • Alternative names

    • hLhx2 antibody
    • Homeobox protein LH-2 antibody
    • Homeobox protein LH2 antibody
    • LH2 antibody
    • LHX2 antibody
    • LHX2_HUMAN antibody
    • LIM homeobox 2 antibody
    • LIM homeobox protein 2 antibody
    • LIM HOX gene 2 antibody
    • LIM/homeobox protein Lhx2 antibody
    see all

Images

  • Western blot - Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
    Western blot - Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
    All lanes : Anti-Lhx2/LH2 antibody [EPR9539] (ab140614) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : T.T. cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)
    Anti-Lhx2/LH2 antibody [EPR9539] (ab140614)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab140614? Please let us know so that we can cite the reference in this datasheet.

ab140614 has been referenced in 1 publication.

  • Liang TS  et al. MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/ß-catenin signaling pathway by down-regulating LHX2. J Exp Clin Cancer Res 38:97 (2019). PubMed: 30791932

Customer reviews and Q&As

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