Product nameAnti-LHX3 / LIM antibody
See all LHX3 / LIM primary antibodies
DescriptionRabbit polyclonal to LHX3 / LIM
SpecificityAb14555 recognizes LHX3 / LIM and does not appear to crossreact with gsh4 on tissue sections.
Tested applicationsSuitable for: ICC/IF, IHC - Wholemount, IHC-P, IHC-Fr, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Fish
C-terminal portion of mouse LHX3 protein.
Previously labelled as LHX3.
Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab14555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/500.
Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.
|IHC - Wholemount||1/4000 - 1/8000.
Use in conjunction with MEMFA as fixative.
|IHC-P||1/250 - 1/1000.|
|IHC-Fr||1/3000 - 1/6000.|
|WB||1/2500 - 1/5000. Predicted molecular weight: 44 kDa.|
FunctionActs as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
Involvement in diseaseDefects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
DomainThe LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
- Information by UniProt
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This product has been referenced in:
- Sagner A et al. Olig2 and Hes regulatory dynamics during motor neuron differentiation revealed by single cell transcriptomics. PLoS Biol 16:e2003127 (2018). Read more (PubMed: 29389974) »
- Yan Y et al. Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2. Nat Commun 6:7006 (2015). IHC . Read more (PubMed: 25943695) »