Overview

  • Product name

  • Description

    Rabbit polyclonal to LHX3/LIM
  • Host species

    Rabbit
  • Specificity

    Ab14555 recognizes LHX3/LIM and does not appear to crossreact with gsh4 on tissue sections.

  • Tested applications

    Suitable for: ICC/IF, IHC - Wholemount, IHC-P, IHC-Fr, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human, Fish
  • Immunogen

    Recombinant fragment corresponding to Mouse LHX3/LIM (C terminal).

  • General notes

    Previously labelled as LHX3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab14555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/500.

Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.

IHC - Wholemount 1/4000 - 1/8000.

Use in conjunction with MEMFA as fixative.

IHC-P 1/250 - 1/1000.
IHC-Fr 1/3000 - 1/6000.
WB 1/2500 - 1/5000. Predicted molecular weight: 44 kDa.
IP 1/200.

Target

  • Function

    Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
  • Involvement in disease

    Defects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Domain

    The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CPHD 3 antibody
    • CPHD3 antibody
    • DKFZp762A2013 antibody
    • LHX 3 antibody
    • LHX3 antibody
    • LHX3_HUMAN antibody
    • LIM 3 antibody
    • LIM homeobox 3 antibody
    • LIM homeobox gene 3 antibody
    • LIM homeobox protein 3 antibody
    • LIM/homeobox protein Lhx3 antibody
    • LIM/homeodomain protein LHX3 antibody
    • Lim3 antibody
    • M2 LHX3 antibody
    • mLim-3 antibody
    • mLIM3 antibody
    • P LIM antibody
    see all

References

This product has been referenced in:

  • Nam H  et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification. Elife 8:N/A (2019). Read more (PubMed: 31305241) »
  • Sagner A  et al. Olig2 and Hes regulatory dynamics during motor neuron differentiation revealed by single cell transcriptomics. PLoS Biol 16:e2003127 (2018). Read more (PubMed: 29389974) »
See all 7 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. I regret to inform you that there are no details available regarding the characterization of ab14555 in Western blotting. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=14555&mode=questionaire

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