Overview

  • Product name
    Anti-LHX6 antibody
    See all LHX6 primary antibodies
  • Description
    Rabbit polyclonal to LHX6
  • Host species
    Rabbit
  • Specificity
    ab22885 recognises LHX6
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, DogDoes not react with: Mouse
  • Immunogen

    Synthetic peptide: RRVIQVWFQN CRARHKKHTP QHPVPPSGAP PSRLPSALSD DIHYTPFSSP, corresponding to a region within C terminal amino acids 289-338 of Human LHX6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HepG2 cell lysate and human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab22885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Probable transcription factor required for the expression of a subset of genes involved in interneurons migration and development. Functions in the specification of cortical interneuron subtypes and in the migration of GABAergic interneuron precursors from the subpallium to the cerebral cortex.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • LHX 6 antibody
      • LHX 6.1 antibody
      • LHX6 antibody
      • LHX6.1 antibody
      • LHX6_HUMAN antibody
      • LIM homeobox 6 antibody
      • LIM homeobox protein 6 antibody
      • LIM homeodomain protein 6.1 antibody
      • LIM/homeobox protein Lhx6 antibody
      • LIM/homeobox protein Lhx6.1 antibody
      • MGC119542 antibody
      • MGC119544 antibody
      • MGC119545 antibody
      • OTTHUMP00000064113 antibody
      • OTTHUMP00000064114 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHX6 antibody (ab22885)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHX6 antibody (ab22885)
      Immunohistochemical analysis of LHX6 expression in paraffin embedded human liver tissue. ab22885 was used at 4 ug/ml. Arrows indicate positively labelled hepatocytes.
    • Western blot - Anti-LHX6 antibody (ab22885)
      Western blot - Anti-LHX6 antibody (ab22885)
      Lane 1 : Anti-LHX6 antibody (ab22885) at 5 µg/ml
      Lane 2 : Anti-LHX6 antibody (ab22885) at 2.5 µg/ml
      Lane 3 : Anti-LHX6 antibody (ab22885) at 1.25 µg/ml

      All lanes : Cell lysate prepared from human HepG2 cells

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 40 kDa
      Observed band size: 50 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • García-Moreno F  et al. A neuronal migratory pathway crossing from diencephalon to telencephalon populates amygdala nuclei. Nat Neurosci 13:680-9 (2010). IHC-P ; Mouse . Read more (PubMed: 20495559) »
    • Jakovcevski I  et al. Multiple Origins of Human Neocortical Interneurons Are Supported by Distinct Expression of Transcription Factors. Cereb Cortex : (2010). IHC-Fr ; Human . Read more (PubMed: 21139075) »

    See all 3 Publications for this product

    Publishing research using ab22885? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    4 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-LHX6 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human fibroblast and stem cells)
    Loading amount
    50 µg
    Specification
    human fibroblast and stem cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Sep 10 2012

    BATCH NUMBER 222072 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Ab identifies MOUSE Lhx6 (aprox. 50KDa band). However, in 293T cells (HUMAN), there are additional bands STRONGER than the 50KDa band and of HIGHER MW (d...

    Read More

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Thank you also for the image that you have provided me with, it is most useful in understanding the difficulties that you have experienced. As...

    Read More

    DESCRIPTION OF THE PROBLEM The Ab recognized three major bands (of 105, 60 and 35 kDa) and a minor one of Mr 50kDa SAMPLE mouse brain PRIMARY ANTIBODY Washed with TPBS and incubation with Rabbit polyclonal antibody antiLhx6 Ab22885 1ug/ml in ...

    Read More

    Thank you for your enquiry. I am sorry to hear you are having a problem with ab22885 (LHX6 antibody). To improve the detection of LHX6, I would like to suggest the following modifications to your protocol: 1) As LHX6 is a nuclear protein, use RI...

    Read More

    Immunohistochemistry (Frozen sections) abreview for Anti-LHX6 antibody

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain, fixed in acrolein)
    Specification
    brain, fixed in acrolein
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 6%
    Read More
    Username

    Miss. Nina Hasen

    Verified customer

    Submitted Jun 01 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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