Product nameAnti-LHX6 antibody
See all LHX6 primary antibodies
DescriptionRabbit polyclonal to LHX6
Tested applicationsSuitable for: WB, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Human
- ab45983 gave a positive signal in Human Brain Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).|
FunctionProbable transcription factor required for the expression of a subset of genes involved in interneurons migration and development. Functions in the specification of cortical interneuron subtypes and in the migration of GABAergic interneuron precursors from the subpallium to the cerebral cortex.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
- Information by UniProt
- LHX 6 antibody
- LHX 6.1 antibody
- LHX6 antibody
Anti-LHX6 antibody (ab45983) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
ab45983 staining LHX6 in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.
This product has been referenced in:
- Aligny C et al. Ketamine alters cortical integration of GABAergic interneurons and induces long-term sex-dependent impairments in transgenic Gad67-GFP mice. Cell Death Dis 5:e1311 (2014). WB ; Mouse . Read more (PubMed: 24991763) »