Overview

  • Product name

  • Description

    Rabbit polyclonal to Lhx8

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species

    Rabbit
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Lhx8.

    Read Abcam's proprietary immunogen policy (Peptide available as ab45116.)

  • Positive control

    • This antibody gave a positive signal in the following Tissue Lysates: Mouse Liver, Rat Liver, Embryonic Mouse Brain and Embryonic Rat Brain.

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.

Target

Images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-Lhx8 antibody (ab41519) at 1 µg/ml

    Lane 1 : E14 Rat Embryo Brain Tissue Lysate
    Lane 2 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 3 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 4 : Mouse brain tissue lysate - total protein (0 days) (ab7188)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 41 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab41519 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • All lanes : Anti-Lhx8 antibody (ab41519) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Shi J  et al. Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro. In Vitro Cell Dev Biol Anim 48:603-9 (2012). IHC . Read more (PubMed: 23150137) »
  • White YA  et al. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat Med 18:413-21 (2012). Read more (PubMed: 22366948) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Embryonic day 19.5 brain tissue)
Permeabilization
No
Specification
Embryonic day 19.5 brain tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.3% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 12 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (neuroblastoma)
Loading amount
300000 cells
Specification
neuroblastoma
Gel Running Conditions
Non-reduced Denaturing (12% Bis-Tris)
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: RT°C

Ann-Christin Schmädicke

Verified customer

Submitted Jan 17 2012

Answer

Vielen Dank für Ihre Email und den Abreview über ab41519. Diese Informationen sind sehr wichtig für andere Forscher und wir schätzen Ihr Feedback sehr. Der Gutscheincode wird gültig sobald ein Abreview eingereicht ist. Ihr Gutscheincode ist somit ab sofort gültig. Bitte bestellen Sie Ihren Antikörper vor dem 12. Dezember, der Gutscheincode läuft danach ab. Ich wünsche Ihnen weiterhin viel Erfolg mit ihrem Projekt.

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