Recombinant
RabMAb

Recombinant Anti-LI Cadherin antibody [EPR3996] (ab109190)

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Overview

  • Product name

    Anti-LI Cadherin antibody [EPR3996]
    See all LI Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR3996] to LI Cadherin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human LI Cadherin aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human small intestine, Human colon cancer, and HT-29 cell lysates, Human colon and Human colonic adenocarcinoma tissues

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 2.90 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3996

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function

      Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.

    • Tissue specificity

      Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.

    • Sequence similarities

      Contains 7 cadherin domains.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • BILL-cadherin antibody
        • CAD17_HUMAN antibody
        • Cadherin 16 antibody
        • Cadherin 16, formerly antibody
        • Cadherin 17 antibody
        • cadherin 17, LI cadherin (liver-intestine) antibody
        • cadherin, liver-intestine antibody
        • Cadherin-17 antibody
        • CDH16 antibody
        • CDH16, formerly antibody
        • Cdh17 antibody
        • FLJ26931 antibody
        • Formerly Cadherin 16 antibody
        • Formerly CDH16 antibody
        • HPT 1 antibody
        • HPT-1 cadherin antibody
        • human intestinal peptide-associated transporter HPT-1 antibody
        • human peptide transporter 1 antibody
        • Intestinal peptide-associated transporter HPT 1 antibody
        • Intestinal peptide-associated transporter HPT-1 antibody
        • LI-cadherin antibody
        • Liver Cadherin antibody
        • liver intestine cadherin antibody
        • Liver-intestine cadherin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

        Immunohistochemical staining of paraffin-embedded Human colon tissue using ab109190 at a dilution of 1/250.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Western blot - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        All lanes : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution

        Lane 1 : Human small intestine cell lysate
        Lane 2 : Human colon cancer cell lysate
        Lane 3 : HT-29 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 92 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

        Immunohistochemical staining of paraffin-embedded Human colonic adenocarcinoma tissue using ab109190 at a dilution of 1/250.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

        ab109190 showing negative staining in Skeletal muscle tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

        ab109190 showing negative staining in Normal kidney tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

        ab109190 showing negative staining in Normal tonsil tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

        ab109190 showing positive staining in Normal colon tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

        ab109190 showing negative staining in Normal brain tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Other - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Other - Anti-LI Cadherin antibody [EPR3996] (ab109190)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab109190 has not yet been referenced specifically in any publications.

      Publishing research using ab109190? Please let us know so that we can cite the reference in this datasheet.

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