Recombinant Anti-LI Cadherin antibody [EPR3996] (ab109190)

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Western blot - Anti-LI Cadherin antibody [EPR3996] (ab109190)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3996] to LI Cadherin
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-LI Cadherin antibody [EPR3996]
See all LI Cadherin primary antibodies
Description

Rabbit monoclonal [EPR3996] to LI Cadherin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human small intestine, Human colon cancer, and HT-29 cell lysates. Human LoVo, small intestine and colon lysates. IHC-P: Human colon and Human colonic adenocarcinoma tissues
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 2.90 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3996
Isotype

IgG
Research areas

Alternative Versions
- Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human LI Cadherin protein (ab114173)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
IHC-P	Human
WB	Human

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC or IP.

Function

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
Tissue specificity

Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.
Sequence similarities

Contains 7 cadherin domains.
Cellular localization

Cell membrane.
Target information above from: UniProt accession Q12864 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1015 Human
- Omim: 603017 Human
- SwissProt: Q12864 Human
- Unigene: 591853 Human
Alternative names
- BILL-cadherin antibody
- CAD17_HUMAN antibody
- Cadherin 16 antibody
- Cadherin 16, formerly antibody
- Cadherin 17 antibody
- cadherin 17, LI cadherin (liver-intestine) antibody
- cadherin, liver-intestine antibody
- Cadherin-17 antibody
- CDH16 antibody
- CDH16, formerly antibody
- Cdh17 antibody
- FLJ26931 antibody
- Formerly Cadherin 16 antibody
- Formerly CDH16 antibody
- HPT 1 antibody
- HPT-1 cadherin antibody
- human intestinal peptide-associated transporter HPT-1 antibody
- human peptide transporter 1 antibody
- Intestinal peptide-associated transporter HPT 1 antibody
- Intestinal peptide-associated transporter HPT-1 antibody
- LI-cadherin antibody
- Liver Cadherin antibody
- liver intestine cadherin antibody
- Liver-intestine cadherin antibody
see all

Western blot - Anti-LI Cadherin antibody [EPR3996] (ab109190)

Lanes 1-3 : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution (Purified)
Lanes 4-5 : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution

Lane 1 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Human small intestine lysate
Lane 3 : Human colon lysate
Lane 4 : Human spleen lysate
Lane 5 : Human kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 92 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?

The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 18552820).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling LI Cadherin with purified ab109190 at 1/300 dilution (0.48 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

Immunohistochemical staining of paraffin-embedded Human colon tissue using ab109190 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-LI Cadherin antibody [EPR3996] (ab109190)

All lanes : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution

Lane 1 : Human small intestine cell lysate
Lane 2 : Human colon cancer cell lysate
Lane 3 : HT-29 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

Immunohistochemical staining of paraffin-embedded Human colonic adenocarcinoma tissue using ab109190 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

ab109190 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

ab109190 showing negative staining in Normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

ab109190 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

ab109190 showing positive staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

ab109190 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-LI Cadherin antibody [EPR3996] (ab109190)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-LI Cadherin antibody [EPR3996] (ab109190)

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet

Publishing research using ab109190? Please let us know so that we can cite the reference in this datasheet.

ab109190 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-LI Cadherin antibody [EPR3996] (ab109190)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (K_D)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (0)

Customer reviews and Q&As

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (0)

Customer reviews and Q&As

Dissociation constant (K_D)