RecombinantRabMAb

Recombinant Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • OI-RD Scanning - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
  • Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3996] to LI Cadherin - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free
    See all LI Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR3996] to LI Cadherin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human LI Cadherin aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: Human small intestine, Human colon cancer, and HT-29 cell lysates. Human LoVo, small intestine and colon lysates. IHC-P: Human colon and Human colonic adenocarcinoma tissues

  • General notes

    ab247786 is the carrier-free version of ab109190 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247786 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 2.90 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3996

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab247786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.

    • Tissue specificity

      Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.

    • Sequence similarities

      Contains 7 cadherin domains.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • BILL-cadherin antibody
        • CAD17_HUMAN antibody
        • Cadherin 16 antibody
        • Cadherin 16, formerly antibody
        • Cadherin 17 antibody
        • cadherin 17, LI cadherin (liver-intestine) antibody
        • cadherin, liver-intestine antibody
        • Cadherin-17 antibody
        • CDH16 antibody
        • CDH16, formerly antibody
        • Cdh17 antibody
        • FLJ26931 antibody
        • Formerly Cadherin 16 antibody
        • Formerly CDH16 antibody
        • HPT 1 antibody
        • HPT-1 cadherin antibody
        • human intestinal peptide-associated transporter HPT-1 antibody
        • human peptide transporter 1 antibody
        • Intestinal peptide-associated transporter HPT 1 antibody
        • Intestinal peptide-associated transporter HPT-1 antibody
        • LI-cadherin antibody
        • Liver Cadherin antibody
        • liver intestine cadherin antibody
        • Liver-intestine cadherin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

        This data was developed using ab109190, the same antibody clone in a different buffer formulation.

        Immunohistochemical staining of paraffin-embedded Human colon tissue using ab109190 at a dilution of 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        All lanes : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution

        Lane 1 : Human small intestine cell lysate
        Lane 2 : Human colon cancer cell lysate
        Lane 3 : HT-29 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 92 kDa



        This data was developed using ab109190, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

        This data was developed using ab109190, the same antibody clone in a different buffer formulation.

        Immunohistochemical staining of paraffin-embedded Human colonic adenocarcinoma tissue using ab109190 at a dilution of 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

        This data was developed using ab109190, the same antibody clone in a different buffer formulation.

        ab109190 showing negative staining in Skeletal muscle tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        All lanes : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution (purified)

        Lane 1 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
        Lane 2 : Human small intestine lysate
        Lane 3 : Human colon lysate
        Lane 4 : Human spleen lysate
        Lane 5 : Human kidney lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 92 kDa
        Observed band size: 120 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab109190, the same antibody clone in a different buffer formulation.

        The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 18552820).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        This data was developed using ab109190, the same antibody clone in a different buffer formulation.
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling LI Cadherin with purified ab109190 at 1/300 dilution (0.48 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        This data was developed using ab109190, the same antibody clone in a different buffer formulation.ab109190 showing negative staining in Normal kidney tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        This data was developed using ab109190, the same antibody clone in a different buffer formulation.ab109190 showing negative staining in Normal tonsil tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        This data was developed using ab109190, the same antibody clone in a different buffer formulation.ab109190 showing positive staining in Normal colon tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        This data was developed using ab109190, the same antibody clone in a different buffer formulation.ab109190 showing negative staining in Normal brain tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • OI-RD Scanning - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        OI-RD Scanning - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        This data was developed using ab109190, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
        Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab247786? Please let us know so that we can cite the reference in this datasheet.

      ab247786 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab247786.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com