Overview

  • Product name

    Anti-LI Cadherin antibody [SP183] - C-terminal
    See all LI Cadherin primary antibodies
  • Description

    Rabbit monoclonal [SP183] to LI Cadherin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human LI Cadherin aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q12864

  • Positive control

    • Human colon, colon adenocarcinoma, breast, prostate and stomach adenocarcinoma tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    SP183
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform Antigen Retrieval by boil tissue sections in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
  • Tissue specificity

    Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.
  • Sequence similarities

    Contains 7 cadherin domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BILL-cadherin antibody
    • CAD17_HUMAN antibody
    • Cadherin 16 antibody
    • Cadherin 16, formerly antibody
    • Cadherin 17 antibody
    • cadherin 17, LI cadherin (liver-intestine) antibody
    • cadherin, liver-intestine antibody
    • Cadherin-17 antibody
    • CDH16 antibody
    • CDH16, formerly antibody
    • Cdh17 antibody
    • FLJ26931 antibody
    • Formerly Cadherin 16 antibody
    • Formerly CDH16 antibody
    • HPT 1 antibody
    • HPT-1 cadherin antibody
    • human intestinal peptide-associated transporter HPT-1 antibody
    • human peptide transporter 1 antibody
    • Intestinal peptide-associated transporter HPT 1 antibody
    • Intestinal peptide-associated transporter HPT-1 antibody
    • LI-cadherin antibody
    • Liver Cadherin antibody
    • liver intestine cadherin antibody
    • Liver-intestine cadherin antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma
    tissue sections labeling LI Cadherin with ab183318 at 1/100 dilution (2.42 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183318 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon
    tissue sections labeling LI Cadherin with ab183318 at 1/100 dilution (2.42 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human colon, performed on a Leica Biosystems BOND™ RX instrument
    The section was incubated with ab183318 for 30 mins at room temperature.

  • Immunohistochemical analysis of Human colon tissue labeling LI Cadherin using ab183318 at a 1/100 dilution.

  • Immunohistochemical analysis of Human colon adenocarcinoma tissue labeling LI Cadherin using ab183318 at a 1/100 dilution.

  • Immunohistochemical analysis of Human stomach adenocarcinoma tissue labeling LI Cadherin using ab183318 at a 1/100 dilution

References

This product has been referenced in:

  • Broede A  et al. Differential diagnosis of bladder versus colorectal adenocarcinoma: keratin 7 and GATA3 positivity in nuclear ß-catenin-negative glandular tumours defines adenocarcinoma of the bladder. J Clin Pathol 69:307-12 (2016). Read more (PubMed: 26463756) »
  • Hebras C & McDougall A Urochordate Ascidians Possess a Single Isoform of Aurora Kinase That Localizes to the Midbody via TPX2 in Eggs and Cleavage Stage Embryos. PLoS One 7:e45431 (2012). IF . Read more (PubMed: 23029005) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183318.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up