Anti-LIAS antibody (ab246917)
Key features and details
- Rabbit polyclonal to LIAS
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LIAS antibody
See all LIAS primary antibodies -
Description
Rabbit polyclonal to LIAS -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human LIAS aa 200-350.
Database link: O43766 -
Positive control
- IHC-P: Human testis, cerebellum, prostate, small intestine and hippocampus tissues; Mouse cerebellum, lateral ventricle and subfornical organ tissues. ICC/IF: U-2 OS cells. WB: NIH 3T3 and NBT-11 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab246917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Relevance
LIAS (Lipoic acid synthetase) is involved in the synthesis of alpha-(+)-lipoic acid. It may have a role in the sulfur insertion chemistry in lipoate biosynthesis. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 530865 Cow
- Entrez Gene: 11019 Human
- Entrez Gene: 79464 Mouse
- Entrez Gene: 305348 Rat
- Omim: 607031 Human
- SwissProt: Q5BIP7 Cow
- SwissProt: O43766 Human
- SwissProt: Q99M04 Mouse
see all -
Alternative names
- HUSSY 01 antibody
- LAS antibody
- LIP 1 antibody
see all
Images
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Formalin-fixed, paraffin-embedded mouse cerebellum tissue stained for LIAS with ab246917 at a 1/50 dilution (green) in immunohistochemical analysis.
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ab246917 staining LIAS in human U-2 OS cells by immunocytochemistry. Cells were fixed with PFA, permeabilized with Triton X-100 and incubated with ab246917 at 0.25 µg/ml.
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Formalin-fixed, paraffin-embedded human small intestine tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
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All lanes : Anti-LIAS antibody (ab246917) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate -
Formalin-fixed, paraffin-embedded human testis tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cerebellum tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human hippocampus tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded mouse lateral ventricle tissue stained for LIAS with ab246917 at a 1/50 dilution (green) in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded mouse subfornical organ tissue stained for LIAS with ab246917 at a 1/50 dilution (green) in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab246917 has not yet been referenced specifically in any publications.