Key features and details
- Rabbit polyclonal to LIAS
- Suitable for: IHC-P, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-LIAS antibody
See all LIAS primary antibodies
DescriptionRabbit polyclonal to LIAS
Tested applicationsSuitable for: IHC-P, WB, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- IHC-P: Human testis, cerebellum, prostate, small intestine and hippocampus tissues; Mouse cerebellum, lateral ventricle and subfornical organ tissues. ICC: U-2 OS cells. WB: NIH 3T3 and NBT-11 cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC||Use a concentration of 0.25 - 2 µg/ml.|
RelevanceLIAS (Lipoic acid synthetase) is involved in the synthesis of alpha-(+)-lipoic acid. It may have a role in the sulfur insertion chemistry in lipoate biosynthesis.
- HUSSY 01 antibody
- LAS antibody
- LIP 1 antibody
Formalin-fixed, paraffin-embedded mouse cerebellum tissue stained for LIAS with ab246917 at a 1/50 dilution (green) in immunohistochemical analysis.
ab246917 staining LIAS in human U-2 OS cells by immunocytochemistry. Cells were fixed with PFA, permeabilized with Triton X-100 and incubated with ab246917 at 0.25 µg/ml.
Formalin-fixed, paraffin-embedded human small intestine tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-LIAS antibody (ab246917) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate
Formalin-fixed, paraffin-embedded human testis tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human prostate tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human hippocampus tissue stained for LIAS with ab246917 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse lateral ventricle tissue stained for LIAS with ab246917 at a 1/50 dilution (green) in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse subfornical organ tissue stained for LIAS with ab246917 at a 1/50 dilution (green) in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246917 has not yet been referenced specifically in any publications.