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    lif-antibody-ab172023.pdf

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Immunology Innate Immunity Cytokines IL6 Related
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Anti-LIF antibody (ab172023)

  • Datasheet
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Western blot - Anti-LIF antibody (ab172023)

    Key features and details

    • Mouse polyclonal to LIF
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Recombinant human LIF protein (ab188058)
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    View more associated products

    Overview

    • Product name

      Anti-LIF antibody
      See all LIF primary antibodies
    • Description

      Mouse polyclonal to LIF
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Full length protein corresponding to Human LIF aa 1-202. (NP_002300.1)
      Sequence:

      MKVLAAGVVPLLLVLHWKHGAGSPLPITPVNATCAIRHPCHNNLMNQIRS QLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKA KLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSN VLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQ AF


      Database link: P15018
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Purchase matching WB positive control:Recombinant human LIF protein
      • LIF transfected 293T lysate
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.4
      Constituent: 100% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • IL6 Related
      • Immunology
      • Innate Immunity
      • Cytokines
      • Other
      • Cell Biology
      • Cell Cycle
      • Cell differentiation
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Cancer
      • Cell cycle
      • Cell differentiation

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)
    • Recombinant Protein

      • Recombinant human LIF protein (ab188058)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab172023 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.

    Target

    • Function

      LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
    • Sequence similarities

      Belongs to the LIF/OSM family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P15018 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3976 Human
      • Omim: 159540 Human
      • SwissProt: P15018 Human
      • Unigene: 2250 Human
      • Alternative names

        • CDF antibody
        • Cholinergic Differentiation Factor antibody
        • D factor antibody
        • DIA antibody
        • Differentiation inducing factor antibody
        • differentiation inhibitory activity antibody
        • Differentiation stimulating factor antibody
        • Differentiation-stimulating factor antibody
        • Emfilermin antibody
        • Hepatocyte stimulating factor III antibody
        • HILDA antibody
        • Human interleukin in DA cells antibody
        • Leukemia inhibitory factor antibody
        • LIF antibody
        • LIF_HUMAN antibody
        • Melanoma derived LPL inhibitor antibody
        • Melanoma-derived LPL inhibitor antibody
        • MLPLI antibody
        see all

      Images

      • Western blot - Anti-LIF antibody (ab172023)
        Western blot - Anti-LIF antibody (ab172023)
        All lanes : Anti-LIF antibody (ab172023) at 1 µg/ml

        Lane 1 : LIF transfected 293T lysate
        Lane 2 : Non-transfected 293T lysate

        Lysates/proteins at 15 µl per lane.

        Predicted band size: 22 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab172023? Please let us know so that we can cite the reference in this datasheet.

      ab172023 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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