Product nameAnti-LIF antibody (Biotin)
See all LIF primary antibodies
DescriptionRabbit polyclonal to LIF (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Predicted to work with: Human
Recombinant full length protein corresponding to Human LIF aa 23-202. Produced in E. coli. Full length mature chain without signal peptide.
SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGE PFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRD QKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDT SGKDVFQKKKLGCQLLGKYKQIIAVLAQAF
Database link: P15018
- WB: Recombinant human LIF protein. sELISA: Recombinant human LIF protein.
FormLyophilised:Reconstitute in sterile PBS + 0.1% BSA to 0.1-1.0mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Concentration information loading...
Our Abpromise guarantee covers the use of ab245854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 22 kDa.
No endogenous data, tested with recombinant protein only.
FunctionLIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Sequence similaritiesBelongs to the LIF/OSM family.
- Information by UniProt
- CDF antibody
- Cholinergic Differentiation Factor antibody
- D factor antibody
To detect human LIF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.
When used in conjunction with compatible development reagents the detection limit for recombinant human LIF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng/lane respectively.
To detect human LIF by sandwich ELISA (using 100 μl/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with a capture antibody,allows the detection of at least 0.2 – 0.4 ng/well of recombinant human LIF.
ab245854 has not yet been referenced specifically in any publications.