Anti-LIF antibody [IMG39N7D10] (ab138002)

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Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)

Key features and details

  • Rat monoclonal [IMG39N7D10] to LIF
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG2b

Overview

  • Product name

    Anti-LIF antibody [IMG39N7D10]
    See all LIF primary antibodies

  • Description

    Rat monoclonal [IMG39N7D10] to LIF

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment

  • Immunogen

    Recombinant full length mature Mouse LIF containing amino acids 24-203 (NP_032527).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Full length recombinant LIF protein, Mouse and Human spleen lysate; Mouse colon tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab138002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 3 - 5 µg/ml. Detects a band of approximately 22-34 kDa (predicted molecular weight: 22 kDa).
IHC-P
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 3 - 5 µg/ml. Detects a band of approximately 22-34 kDa (predicted molecular weight: 22 kDa).
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Function

    LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

  • Sequence similarities

    Belongs to the LIF/OSM family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • CDF antibody
      • Cholinergic Differentiation Factor antibody
      • D factor antibody
      • DIA antibody
      • Differentiation inducing factor antibody
      • differentiation inhibitory activity antibody
      • Differentiation stimulating factor antibody
      • Differentiation-stimulating factor antibody
      • Emfilermin antibody
      • Hepatocyte stimulating factor III antibody
      • HILDA antibody
      • Human interleukin in DA cells antibody
      • Leukemia inhibitory factor antibody
      • LIF antibody
      • LIF_HUMAN antibody
      • Melanoma derived LPL inhibitor antibody
      • Melanoma-derived LPL inhibitor antibody
      • MLPLI antibody
      see all

    Images

    • Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)
      Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)
      All lanes : Anti-LIF antibody [IMG39N7D10] (ab138002) at 3 µg

      Lane 1 : Full length recombinant LIF protein
      Lane 2 : Mouse spleen lysate
      Lane 3 : Human spleen lysate

      Secondary
      All lanes : Goat anti-Rat Ig HRP

      Developed using the ECL technique.

      Predicted band size: 22 kDa



      The LIF protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)
      Immunohistochemical analysis of LIF in Mouse colon tissue probed with ab138002 at 5 µg/ml.

    References (5)

    Publishing research using ab138002? Please let us know so that we can cite the reference in this datasheet.

    ab138002 has been referenced in 5 publications.

    • Yu Y  et al. ß-Sitosterol Ameliorates Endometrium Receptivity in PCOS-Like Mice: The Mediation of Gut Microbiota. Front Nutr 8:667130 (2021). PubMed: 34179058
    • Yi KW  et al. Bone marrow-derived cells or C-X-C motif chemokine 12 (CXCL12) treatment improve thin endometrium in a mouse model. Biol Reprod 100:61-70 (2019). PubMed: 30084961
    • Guo S  et al. GnRH agonist improves pregnancy outcome in mice with induced adenomyosis by restoring endometrial receptivity. Drug Des Devel Ther 12:1621-1631 (2018). PubMed: 29922037
    • Yuan M  et al. Environmentally relevant levels of bisphenol A affect uterine decidualization and embryo implantation through the estrogen receptor/serum and glucocorticoid-regulated kinase 1/epithelial sodium ion channel a-subunit pathway in a mouse model. Fertil Steril 109:735-744.e1 (2018). PubMed: 29605410
    • Sadlowski C  et al. Graphene-based biosensor for on-chip detection of bio-orthogonally labeled proteins to identify the circulating biomarkers of aging during heterochronic parabiosis. Lab Chip 18:3230-3238 (2018). PubMed: 30239548

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