Anti-LIF antibody [IMG39N7D10] (ab138002)
![Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)](https://www.abcam.com/ps/products/138/ab138002/Images/ab138002-153292-anti-lif-antibody-img39n7d10-western-blot.jpg "Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)")
Key features and details
- Rat monoclonal [IMG39N7D10] to LIF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-LIF antibody [IMG39N7D10]
See all LIF primary antibodies -
Description
Rat monoclonal [IMG39N7D10] to LIF -
Host species
Rat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant full length mature Mouse LIF containing amino acids 24-203 (NP_032527).
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Positive control
- Full length recombinant LIF protein, Mouse and Human spleen lysate; Mouse colon tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
IMG39N7D10 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab138002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 3 - 5 µg/ml. Detects a band of approximately 22-34 kDa (predicted molecular weight: 22 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. |
Target
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Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. -
Sequence similarities
Belongs to the LIF/OSM family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3976 Human
- Entrez Gene: 16878 Mouse
- Omim: 159540 Human
- SwissProt: P15018 Human
- SwissProt: P09056 Mouse
- Unigene: 2250 Human
- Unigene: 4964 Mouse
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Alternative names
- CDF antibody
- Cholinergic Differentiation Factor antibody
- D factor antibody
see all
Images
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Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)All lanes : Anti-LIF antibody [IMG39N7D10] (ab138002) at 3 µg
Lane 1 : Full length recombinant LIF protein
Lane 2 : Mouse spleen lysate
Lane 3 : Human spleen lysate
Secondary
All lanes : Goat anti-Rat Ig HRP
Developed using the ECL technique.
Predicted band size: 22 kDa
The LIF protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)](https://www.abcam.com/ps/products/138/ab138002/Images/ab138002-153291-anti-lif-antibody-img39n7d10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)Immunohistochemical analysis of LIF in Mouse colon tissue probed with ab138002 at 5 µg/ml.
Protocols
Datasheets and documents
References (4)
ab138002 has been referenced in 4 publications.
- Yi KW et al. Bone marrow-derived cells or C-X-C motif chemokine 12 (CXCL12) treatment improve thin endometrium in a mouse model. Biol Reprod 100:61-70 (2019). PubMed: 30084961
- Guo S et al. GnRH agonist improves pregnancy outcome in mice with induced adenomyosis by restoring endometrial receptivity. Drug Des Devel Ther 12:1621-1631 (2018). PubMed: 29922037
- Yuan M et al. Environmentally relevant levels of bisphenol A affect uterine decidualization and embryo implantation through the estrogen receptor/serum and glucocorticoid-regulated kinase 1/epithelial sodium ion channel a-subunit pathway in a mouse model. Fertil Steril 109:735-744.e1 (2018). PubMed: 29605410
- Sadlowski C et al. Graphene-based biosensor for on-chip detection of bio-orthogonally labeled proteins to identify the circulating biomarkers of aging during heterochronic parabiosis. Lab Chip 18:3230-3238 (2018). PubMed: 30239548
