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Immunology Innate Immunity Cytokines IL6 Related
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Anti-LIF antibody [IMG39N7D10] (ab138002)

  • Datasheet
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Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)

Key features and details

  • Rat monoclonal [IMG39N7D10] to LIF
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-LIF antibody [IMG39N7D10]
    See all LIF primary antibodies
  • Description

    Rat monoclonal [IMG39N7D10] to LIF
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment
  • Immunogen

    Recombinant full length mature Mouse LIF containing amino acids 24-203 (NP_032527).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Full length recombinant LIF protein, Mouse and Human spleen lysate; Mouse colon tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    IMG39N7D10
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • IL6 Related
    • Immunology
    • Innate Immunity
    • Cytokines
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Cell cycle
    • Cell differentiation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab7937)
  • Recombinant Protein

    • Recombinant human LIF protein (ab188058)
  • Related Products

    • Recombinant Mouse LIF protein (ab123757)

Applications

Our Abpromise guarantee covers the use of ab138002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 3 - 5 µg/ml. Detects a band of approximately 22-34 kDa (predicted molecular weight: 22 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
  • Sequence similarities

    Belongs to the LIF/OSM family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P15018 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3976 Human
    • Entrez Gene: 16878 Mouse
    • Omim: 159540 Human
    • SwissProt: P15018 Human
    • SwissProt: P09056 Mouse
    • Unigene: 2250 Human
    • Unigene: 4964 Mouse
    • Alternative names

      • CDF antibody
      • Cholinergic Differentiation Factor antibody
      • D factor antibody
      • DIA antibody
      • Differentiation inducing factor antibody
      • differentiation inhibitory activity antibody
      • Differentiation stimulating factor antibody
      • Differentiation-stimulating factor antibody
      • Emfilermin antibody
      • Hepatocyte stimulating factor III antibody
      • HILDA antibody
      • Human interleukin in DA cells antibody
      • Leukemia inhibitory factor antibody
      • LIF antibody
      • LIF_HUMAN antibody
      • Melanoma derived LPL inhibitor antibody
      • Melanoma-derived LPL inhibitor antibody
      • MLPLI antibody
      see all

    Images

    • Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)
      Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)
      All lanes : Anti-LIF antibody [IMG39N7D10] (ab138002) at 3 µg

      Lane 1 : Full length recombinant LIF protein
      Lane 2 : Mouse spleen lysate
      Lane 3 : Human spleen lysate

      Secondary
      All lanes : Goat anti-Rat Ig HRP

      Developed using the ECL technique.

      Predicted band size: 22 kDa



      The LIF protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)
      Immunohistochemical analysis of LIF in Mouse colon tissue probed with ab138002 at 5 µg/ml.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab138002? Please let us know so that we can cite the reference in this datasheet.

    ab138002 has been referenced in 4 publications.

    • Yi KW  et al. Bone marrow-derived cells or C-X-C motif chemokine 12 (CXCL12) treatment improve thin endometrium in a mouse model. Biol Reprod 100:61-70 (2019). PubMed: 30084961
    • Guo S  et al. GnRH agonist improves pregnancy outcome in mice with induced adenomyosis by restoring endometrial receptivity. Drug Des Devel Ther 12:1621-1631 (2018). PubMed: 29922037
    • Yuan M  et al. Environmentally relevant levels of bisphenol A affect uterine decidualization and embryo implantation through the estrogen receptor/serum and glucocorticoid-regulated kinase 1/epithelial sodium ion channel a-subunit pathway in a mouse model. Fertil Steril 109:735-744.e1 (2018). PubMed: 29605410
    • Sadlowski C  et al. Graphene-based biosensor for on-chip detection of bio-orthogonally labeled proteins to identify the circulating biomarkers of aging during heterochronic parabiosis. Lab Chip 18:3230-3238 (2018). PubMed: 30239548

    Customer reviews and Q&As

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