Overview

  • Product name

  • Description

    Rabbit polyclonal to LIFR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Human, Pig, Chimpanzee, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Mouse LIFR aa 500-600 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab130816)

  • Positive control

    • This antibody gave a positive signal in Mouse Placenta tissue lysate and in mouse placenta FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab101228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).

Target

  • Function

    Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
  • Involvement in disease

    Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS) [MIM:601559]; also knowns as Schwartz-Jampel syndrome type 2 (SJS2). SWS is a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias. SWS is characterized by bowing of the lower limbs, with internal cortical thickening, wide metaphyses with abnormal trabecular pattern, and camptodactyly. Additional features include feeding and swallowing difficulties, as well as respiratory distress and hyperthermic episodes, which cause death in the first months of life. The rare survivors develop progressive scoliosis, spontaneous fractures, bowing of the lower limbs, with prominent joints and dysautonomia symptoms, including temperature instability, absent corneal and patellar reflexes, and smooth tongue.
    Note=A chromosomal aberration involving LIFR is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(5;8)(p13;q12) with PLAG1.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 6 fibronectin type-III domains.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD118 antibody
    • CD118 antigen antibody
    • FLJ98106 antibody
    • FLJ99923 antibody
    • Leukemia inhibitory factor receptor alpha antibody
    • Leukemia inhibitory factor receptor antibody
    • LIF R antibody
    • LIF receptor antibody
    • LIF-R antibody
    • Lifr antibody
    • LIFR_HUMAN antibody
    • SJS2 antibody
    • STWS antibody
    • SWS antibody
    see all

Images

  • Anti-LIFR antibody (ab101228) at 1 µg/ml + Mouse Placenta Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 122 kDa
    Observed band size: 122 kDa
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds
  • IHC image of LIFR staining in mouse placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101228, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Wang J  et al. miR-629-3p may serve as a novel biomarker and potential therapeutic target for lung metastases of triple-negative breast cancer. Breast Cancer Res 19:72 (2017). WB ; Mouse . Read more (PubMed: 28629464) »
  • Subramani E  et al. Dysregulated leukemia inhibitory factor and its receptor regulated signal transducers and activators of transcription 3 pathway: a possible cause for repeated implantation failure in women with dormant genital tuberculosis? Fertil Steril 105:1076-1084.e5 (2016). Read more (PubMed: 26776907) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Colon
Blocking step
UV inhibitor - Ventana as blocking agent for 4 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 05 2016

Answer

Ich verstehe Ihre Bedenken.

Es tut mir leid, dass ich Ihnen in diesem Fall nicht mehr helfen konnte.

Ich wünsche Ihnen alles Gute bei der Suche und viel Erfolg mit Ihrem Experiment.

Read More

Question
Answer

Sorry for the delay in getting back to you.

I now have some further information to share with you in regards to the antibody ab89792. This antibody is a mouse monoclonal which has been raised against a recombinant fragment of the human LIFR protein, from residues 45 to 833 (SwissProt reference P42702). This recombinant protein was also used as the positive control when verifying this antibody, giving a band at approximately 110 kDa.

I think the bands observed by your colleague are as would be expected. The full length protein is predicted to be 124 kDa, but this does not take into account the glycosylation which the protein undergoes. I have found reference to the protein being detected at ˜160 kDa in murine samples where a size of ˜123 kDa is predicted from the SwissProt reference P42703:

http://www.reproduction-online.org/content/131/2/341.full#ref-31

Unfortunately, I have had some difficulty intrying to find further publications where the size of the receptor detected through Western blotting is mentioned. However I think the band at 150 kDa could well be the glycosylated, membrane bound LIFR protein.

The band observed at 100 kDa could correspond to the soluble LIFR protein. The soluble protein encompasses residues 1-833. I was again unable to find direct references to the size of protein which has been detected but the size of the protein sequence alone would give 94 kDa. As I think the N-terminal signalling sequence (residues 1-45) is sometimes cleaved from the soluble protein this would reduce this size somewhat, but again with glycosylation you can expect the size to increase. I therefore think a size around 100-110 kDa would be expected.

I am sorry but I will not be able to provide you with a blocking peptide as the antibody is raised against the recombinant protein and the exact binding epitope of the antibody is not known. If you would be interested, I could offer you a testing discount on trying out other anti-LIFR antibody (ab101228). This antibody has not been tested with human samples as yet but as the immunogen used shares 100% homology with the human protein I would expect the antibody to be able to detect the human protein. Withthis antibody I could enquire if we would be able to sell the blocking peptide as well as the antibody if you would like.

I think we have discussed this offer before but here is the link for your information again:

https://www.abcam.com/collaboratordiscount

I hope this information has been of help, if you have any further questions, please do not hesitate to contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up