Overview

  • Product name

    Anti-LIFR antibody [MM0455-9B23]
    See all LIFR primary antibodies
  • Description

    Mouse monoclonal [MM0455-9B23] to LIFR
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Extracellular domain of Human recombinant LIFR protein

Properties

Applications

Our Abpromise guarantee covers the use of ab89792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 124 kDa.
Flow Cyt 1/50 - 1/200.

Target

  • Function

    Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
  • Involvement in disease

    Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS) [MIM:601559]; also knowns as Schwartz-Jampel syndrome type 2 (SJS2). SWS is a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias. SWS is characterized by bowing of the lower limbs, with internal cortical thickening, wide metaphyses with abnormal trabecular pattern, and camptodactyly. Additional features include feeding and swallowing difficulties, as well as respiratory distress and hyperthermic episodes, which cause death in the first months of life. The rare survivors develop progressive scoliosis, spontaneous fractures, bowing of the lower limbs, with prominent joints and dysautonomia symptoms, including temperature instability, absent corneal and patellar reflexes, and smooth tongue.
    Note=A chromosomal aberration involving LIFR is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(5;8)(p13;q12) with PLAG1.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 6 fibronectin type-III domains.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD118 antibody
    • CD118 antigen antibody
    • FLJ98106 antibody
    • FLJ99923 antibody
    • Leukemia inhibitory factor receptor alpha antibody
    • Leukemia inhibitory factor receptor antibody
    • LIF R antibody
    • LIF receptor antibody
    • LIF-R antibody
    • Lifr antibody
    • LIFR_HUMAN antibody
    • SJS2 antibody
    • STWS antibody
    • SWS antibody
    see all

References

This product has been referenced in:

  • Li X  et al. miR-377-3p regulates adipogenic differentiation of human bone marrow mesenchymal stem cells by regulating LIFR. Mol Cell Biochem N/A:N/A (2018). Read more (PubMed: 29959592) »
See 1 Publication for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer


Hela cells were used to test this antibody in flow cytometry. As the immunogen of the antibody is extracellular domain, a regular flow cytometry protocol (without membrane permeabilization is needed) was applied.

Our recommended protocol for indirect flow cytometry can be found at: https://www.abcam.com/index.html?pageconfig=resource&rid=11381

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Answer

Ich verstehe Ihre Bedenken.

Es tut mir leid, dass ich Ihnen in diesem Fall nicht mehr helfen konnte.

Ich wünsche Ihnen alles Gute bei der Suche und viel Erfolg mit Ihrem Experiment.

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Question
Answer

Sorry for the delay in getting back to you.

I now have some further information to share with you in regards to the antibody ab89792. This antibody is a mouse monoclonal which has been raised against a recombinant fragment of the human LIFR protein, from residues 45 to 833 (SwissProt reference P42702). This recombinant protein was also used as the positive control when verifying this antibody, giving a band at approximately 110 kDa.

I think the bands observed by your colleague are as would be expected. The full length protein is predicted to be 124 kDa, but this does not take into account the glycosylation which the protein undergoes. I have found reference to the protein being detected at ˜160 kDa in murine samples where a size of ˜123 kDa is predicted from the SwissProt reference P42703:

http://www.reproduction-online.org/content/131/2/341.full#ref-31

Unfortunately, I have had some difficulty intrying to find further publications where the size of the receptor detected through Western blotting is mentioned. However I think the band at 150 kDa could well be the glycosylated, membrane bound LIFR protein.

The band observed at 100 kDa could correspond to the soluble LIFR protein. The soluble protein encompasses residues 1-833. I was again unable to find direct references to the size of protein which has been detected but the size of the protein sequence alone would give 94 kDa. As I think the N-terminal signalling sequence (residues 1-45) is sometimes cleaved from the soluble protein this would reduce this size somewhat, but again with glycosylation you can expect the size to increase. I therefore think a size around 100-110 kDa would be expected.

I am sorry but I will not be able to provide you with a blocking peptide as the antibody is raised against the recombinant protein and the exact binding epitope of the antibody is not known. If you would be interested, I could offer you a testing discount on trying out other anti-LIFR antibody (ab101228). This antibody has not been tested with human samples as yet but as the immunogen used shares 100% homology with the human protein I would expect the antibody to be able to detect the human protein. Withthis antibody I could enquire if we would be able to sell the blocking peptide as well as the antibody if you would like.

I think we have discussed this offer before but here is the link for your information again:

https://www.abcam.com/collaboratordiscount

I hope this information has been of help, if you have any further questions, please do not hesitate to contact us again.

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Answer

Thank you for contacting us and sorry for the delay in getting back to you.

I am going to have a look into the antibody ab89792 for you to see what I can suggest. I am not sure I will be able to provide the blocking peptide to this antibody but I will certainly try to ascertain exactly what size of protein can be expected. Although the predicted size from SwissProt reference is P42702 indeed 124 kDa but the protein looks like it might go through several post-translational glycosylation events and the size may therefore increase. I will alsotry to find out if this antibody is likely to detect the soluble form of the protein.

Once I have this information to share with you I will get back to you. I am sorry for the delay and any inconvenience this causes you and your colleague.

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Question
Answer

Thank you for confirming those details.

As requested, I have generated the testing discount code for you as below:

Discount code: xxxxxxx


Expiration date: 5th November 2012

Please orderab89792 as normalandquote this discountcode in the "Additional comments" section when submitting your resultsin the Abreview for ab89792.Once the Abreview has been submitted the code will become activated and you can quote the code when placing the order for your free product. This code expires on the 5th November 2012. More information on the testing discount scheme can be found form the following link:

https://www.abcam.com/collaboratordiscount

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Answer

Thank you for contacting us.

Unfortunately, due to the size of our catalogue and the logistics it would involve, we are unable to offer samples sizes of any of out products. However, if you would like to participate in the testing discount scheme I would be happy to generate the testing discount code for you. This scheme means that if you purchase ab89792 as normal,use it with your samples in Western blotting, then share the results (as well as an image) through an Abreview we can offer you afree primary antibody as a reward. More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

Alternatively, if you would like to test ab101228 instead you would also be eligible for a testing discount. Although this antibody has only been tested in mouse as yet, the immunogen used to raise theantibody shares 100% homology to the human protein and would therefore be predicted to react.

If you would be interested in participating in this scheme with either of these antibodiesplease do let me know as a discount code needs to be issued prior to the purchase of ab89792 or ab101228.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Question
Answer

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab89792 Anti-LIFR antibody [MM0455-9B23] in IHC zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer XXXXX, deckt den Wert von ab89792 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in IHC eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount


Als Positivkontrolle eignet sich laut des Human Protein Atlas Gewebe folgende Gewebe: Lymphknoten (Non-Germinal- Center cells), Gewebe aus dem Verdauungstrakt (Speicheldrüsen, Magen, Duodenum, Appendix, Colon und Rektum: Glandular Zellen sollten eine kräftige Färbung aufweisen), Lunge (Makrophagen), Schilddrüse, Nebenschilddrüse und und Nebenniere (Glandulare Zellen).

http://www.proteinatlas.org/ENSG00000113594/normal

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Question
Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde der ab89792 Anti-LIFR antibody [MM0455-9B23] bisher noch nicht in IHC getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab89792 abdecken, und gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab89792 in IHC testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in IHC.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen primären Antikörpers zu dem gleichen Preis wie ab89792 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab89792 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount



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Question
Answer

Thank you for contacting us and your interest in our products.


I have looked into whether the Anti-LIFR antibody (ab89792) could be successfully used with mouse samples. The antibody is raised against the extracellular domain of human LIFR protein (residues 45-833, SwissProt reference P42702). More precise epitope mapping of this antibody has not been performed. Unfortunately, this region only shares 71 % homology with the mouse protein, we could therefore not beconfident that the antibody would be able to recognise the mouse protein.


Unfortunately we do not have any further antibodies to offer against this target currently.



I am sorry that I could not be of more help in this instance. If you need any further information please do not hesitate to contact us again.

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