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I want to know what kind of cells you used to test the quality of lifr antibody for flowcytometry.
Asked on Sep 03 2013
Hela cells were used to test this antibody in flow cytometry. As the immunogen of the antibody is extracellular domain, a regular flow cytometry protocol (without membrane permeabilization is needed) was applied.
Our recommended protocol for indirect flow cytometry can be found at: https://www.abcam.com/index.html?pageconfig=resource&rid=11381
Elisa ThomasAbcam Scientific Support
Answered on Sep 03 2013