Anti-LIG1 antibody (ab238157)
Key features and details
- Rabbit polyclonal to LIG1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-LIG1 antibody
See all LIG1 primary antibodies -
Description
Rabbit polyclonal to LIG1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
-
Immunogen
Recombinant full length protein corresponding to Human LIG1 aa 1 to the C-terminus.
Database link: P18858-1 -
Positive control
- IHC-P: Human placenta tissue. ICC/IF: A549 cells.
-
General notes
This product was previously labelled as DNA Ligase I
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/20 - 1/200.
|
|
| ICC/IF |
1/50 - 1/200.
|
| Notes |
|---|
|
IHC-P
1/20 - 1/200. |
|
ICC/IF
1/50 - 1/200. |
Target
-
Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. -
Sequence similarities
Belongs to the ATP-dependent DNA ligase family. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 3978 Human
- Omim: 126391 Human
- SwissProt: P18858 Human
- Unigene: 1770 Human
-
Alternative names
- DNA ligase 1 antibody
- DNA ligase I antibody
- DNLI1_HUMAN antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody (ab238157)
Paraffin-embedded human placenta tissue stained for LIG1 with ab238157 at a 1/100 dilution in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody (ab238157)A549 (Human lung carcinoma cell line) cells stained for LIG1 (Green) using ab238157 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab238157 has not yet been referenced specifically in any publications.