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    lig1-antibody-ab238157.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis DNA Ligases
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Anti-LIG1 antibody (ab238157)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody (ab238157)
  • Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody (ab238157)

Key features and details

  • Rabbit polyclonal to LIG1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-LIG1 antibody
    See all LIG1 primary antibodies
  • Description

    Rabbit polyclonal to LIG1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human LIG1 aa 1 to the C-terminus.
    Database link: P18858-1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human placenta tissue. ICC/IF: A549 cells.
  • General notes

     This product was previously labelled as DNA Ligase I

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • DNA Ligases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
IHC-P
1/20 - 1/200.
ICC/IF
1/50 - 1/200.
Notes
IHC-P
1/20 - 1/200.
ICC/IF
1/50 - 1/200.

Target

  • Function

    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
  • Sequence similarities

    Belongs to the ATP-dependent DNA ligase family.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P18858 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3978 Human
    • Omim: 126391 Human
    • SwissProt: P18858 Human
    • Unigene: 1770 Human
    • Alternative names

      • DNA ligase 1 antibody
      • DNA ligase I antibody
      • DNLI1_HUMAN antibody
      • LIG 1 antibody
      • lig1 antibody
      • Ligase I DNA ATP dependent antibody
      • MGC117397 antibody
      • MGC130025 antibody
      • Polydeoxyribonucleotide synthase [ATP] 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody (ab238157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody (ab238157)

      Paraffin-embedded human placenta tissue stained for LIG1 with ab238157 at a 1/100 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody (ab238157)
      Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody (ab238157)

      A549 (Human lung carcinoma cell line) cells stained for LIG1 (Green) using ab238157 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab238157? Please let us know so that we can cite the reference in this datasheet.

    ab238157 has not yet been referenced specifically in any publications.

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