Anti-LIG1 antibody (ab238157)
Key features and details
- Rabbit polyclonal to LIG1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LIG1 antibody
See all LIG1 primary antibodies -
Description
Rabbit polyclonal to LIG1 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P Human -
Immunogen
Recombinant full length protein corresponding to Human LIG1 aa 1 to the C-terminus.
Database link: P18858-1 -
Positive control
- IHC-P: Human placenta tissue. ICC/IF: A549 cells.
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General notes
This product was previously labelled as DNA Ligase I
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
IHC-P |
Human
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
|
|
ICC/IF |
1/50 - 1/200.
|
Notes |
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IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. -
Sequence similarities
Belongs to the ATP-dependent DNA ligase family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3978 Human
- Omim: 126391 Human
- SwissProt: P18858 Human
- Unigene: 1770 Human
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Alternative names
- DNA ligase 1 antibody
- DNA ligase I antibody
- DNLI1_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody (ab238157)
Paraffin-embedded human placenta tissue stained for LIG1 with ab238157 at a 1/100 dilution in immunohistochemical analysis.
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A549 (Human lung carcinoma cell line) cells stained for LIG1 (Green) using ab238157 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab238157 has not yet been referenced specifically in any publications.