Anti-LIG1 antibody (ab238157)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-LIG1 antibody
See all LIG1 primary antibodies -
Description
Rabbit polyclonal to LIG1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human LIG1 aa 1-919.
Sequence:MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESD SPVKRPGRKAARVLGSEGEEEDEALSPAKGQKPALDCSQVSPPRPATSPE NNASLSDTSPMDSSPSGIPKRRTARKQLPKRTIQEVLEEQSEDEDREAKR KKEEEEEETPKESLTEAEVATEKEGEDGDQPTTPPKPLKTSKAETPTESV SEPEVATKQELQEEEEQTKPPRRAPKTLSSFFTPRKPAVKKEVKEEEPGA PGKEGAAEGPLDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIE EVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELGVG DGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPPLT ASGVFSKFRDIARLTGSASTAKKIDIIKGLFVACRHSEARFIARSLSGRL RLGLAEQSVLAALSQAVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQG MILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRG ISEVLKRFEEAAFTCEYKYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPD IISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASE IQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLD TKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYL DGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGF SDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLS LSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQ SQIQNQQGEDSGSDPEDTY
Database link: P18858-1 -
Positive control
- IHC-P: Human placenta tissue. ICC/IF: A549 cells.
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General notes
This product was previously labelled as DNA Ligase I
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab238157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. -
Sequence similarities
Belongs to the ATP-dependent DNA ligase family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3978 Human
- Omim: 126391 Human
- SwissProt: P18858 Human
- Unigene: 1770 Human
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Alternative names
- DNA ligase 1 antibody
- DNA ligase I antibody
- DNLI1_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody (ab238157)Paraffin-embedded human placenta tissue stained for LIG1 with ab238157 at a 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody (ab238157)A549 (Human lung carcinoma cell line) cells stained for LIG1 (Green) using ab238157 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
References
ab238157 has not yet been referenced specifically in any publications.
