Recombinant Anti-LIG1 antibody [EPR12464] (ab177946)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12464] to LIG1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
Overview
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Product name
Anti-LIG1 antibody [EPR12464]
See all LIG1 primary antibodies -
Description
Rabbit monoclonal [EPR12464] to LIG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Rat, Human
Does not react with: Mouse -
Immunogen
Synthetic peptide within Human LIG1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P18858 -
Positive control
- WB: HeLa, Jurkat and Molt4 cell lysates IHC-P: Human breast and thyroid gland tissues ICC/IF: HeLa cells.
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General notes
This product was previously labelled as DNA Ligase I
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12464 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab177946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 102 kDa. | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | 1/100 - 1/250. |
Target
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Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. -
Sequence similarities
Belongs to the ATP-dependent DNA ligase family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3978 Human
- Entrez Gene: 81513 Rat
- Omim: 126391 Human
- SwissProt: P18858 Human
- SwissProt: Q9JHY8 Rat
- Unigene: 1770 Human
- Unigene: 20467 Rat
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Alternative names
- DNA ligase 1 antibody
- DNA ligase I antibody
- DNLI1_HUMAN antibody
see all
Images
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All lanes : Anti-LIG1 antibody [EPR12464] (ab177946) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Molt 4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (ab177946)
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling LIG1 with ab177946 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (ab177946)
Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling LIG1 with ab177946 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling LIG1 with ab177946 at 1/100 dilution.
Protocols
References (3)
ab177946 has been referenced in 3 publications.
- Naumann M et al. Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation. Nat Commun 9:335 (2018). WB . PubMed: 29362359
- Maffucci P et al. Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies. J Clin Invest 128:5489-5504 (2018). PubMed: 30395541
- Han J et al. BRCA2 antagonizes classical and alternative nonhomologous end-joining to prevent gross genomic instability. Nat Commun 8:1470 (2017). PubMed: 29133916