Recombinant Anti-LIG1 antibody [EPR12464] (ab177946)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12464] to LIG1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LIG1 antibody [EPR12464]
See all LIG1 primary antibodies -
Description
Rabbit monoclonal [EPR12464] to LIG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: RatDoes not react with: Mouse -
Immunogen
Synthetic peptide within Human LIG1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P18858 -
Positive control
- WB: HeLa, Jurkat and Molt4 cell lysates IHC-P: Human breast and thyroid gland tissues ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12464 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
|
IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
ICC/IF |
1/100 - 1/250.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. -
Sequence similarities
Belongs to the ATP-dependent DNA ligase family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3978 Human
- Entrez Gene: 81513 Rat
- Omim: 126391 Human
- SwissProt: P18858 Human
- SwissProt: Q9JHY8 Rat
- Unigene: 1770 Human
- Unigene: 20467 Rat
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Alternative names
- DNA ligase 1 antibody
- DNA ligase I antibody
- DNLI1_HUMAN antibody
see all
Images
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All lanes : Anti-LIG1 antibody [EPR12464] (ab177946) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Molt 4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa -
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling LIG1 with ab177946 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling LIG1 with ab177946 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling LIG1 with ab177946 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab177946 has been referenced in 5 publications.
- Alblihy A et al. Targeting Mre11 overcomes platinum resistance and induces synthetic lethality in XRCC1 deficient epithelial ovarian cancers. NPJ Precis Oncol 6:51 (2022). PubMed: 35853939
- Li PF et al. Downregulation of DNA ligases in trophoblasts contributes to recurrent pregnancy loss through inducing DNA damages. Placenta 106:7-14 (2021). PubMed: 33601221
- Naumann M et al. Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation. Nat Commun 9:335 (2018). WB . PubMed: 29362359
- Maffucci P et al. Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies. J Clin Invest 128:5489-5504 (2018). PubMed: 30395541
- Han J et al. BRCA2 antagonizes classical and alternative nonhomologous end-joining to prevent gross genomic instability. Nat Commun 8:1470 (2017). PubMed: 29133916