Overview

  • Product name

    Anti-LIG1 antibody [EPR12464]
    See all LIG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12464] to LIG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide within Human LIG1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P18858

  • Positive control

    • WB: HeLa, Jurkat and Molt4 cell lysates IHC-P: Human breast and thyroid gland tissues ICC/IF: HeLa cells.
  • General notes

     

     

     This product was previously labelled as DNA Ligase I

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12464
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • All lanes : Anti-LIG1 antibody [EPR12464] (ab177946) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Molt 4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 102 kDa

    • Immunohistochemical analysis of paraffin-embedded human breast tissue labeling LIG1 with ab177946 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling LIG1 with ab177946 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunofluorescent analysis of HeLa cells labeling LIG1 with ab177946 at 1/100 dilution.

    References

    This product has been referenced in:

    • Naumann M  et al. Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation. Nat Commun 9:335 (2018). WB . Read more (PubMed: 29362359) »
    • Maffucci P  et al. Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies. J Clin Invest 128:5489-5504 (2018). Read more (PubMed: 30395541) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreatic cancer cell line xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
    Permeabilization
    No
    Specification
    Pancreatic cancer cell line xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 23 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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