Overview

  • Product name

  • Description

    Rabbit polyclonal to LILRB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human LILRB1 aa 483-650.
    Database link: Q8NHL6

  • Positive control

    • WB: THP-1 whole cell lysate. Rat brain tissue lysate. IHC-P: Human tonsil tissue. ICC/IF: HEK-293 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500.
WB 1/500 - 1/5000.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
  • Tissue specificity

    Expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells.
  • Sequence similarities

    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD85 antibody
    • CD85 antigen antibody
    • CD85 antigen like family member J antibody
    • CD85 antigen-like family member J antibody
    • CD85j antibody
    • CD85j antigen antibody
    • Ig like transcript 2 antibody
    • ILT 2 antibody
    • ILT-2 antibody
    • ILT2 antibody
    • immunoglobulin like transcript 2 antibody
    • Immunoglobulin-like transcript 2 antibody
    • leukocyte Ig like receptor 1 antibody
    • Leukocyte immunoglobulin like receptor 1 antibody
    • leukocyte immunoglobulin like receptor subfamily B member 1 antibody
    • Leukocyte immunoglobulin like receptor subfamily B member 1 precursor antibody
    • leukocyte immunoglobulin like receptor subfamily B member 1 soluble isoform antibody
    • leukocyte immunoglobulin like receptor, subfamily B, member 1 antibody
    • Leukocyte immunoglobulin-like receptor 1 antibody
    • Leukocyte immunoglobulin-like receptor subfamily B member 1 antibody
    • leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform antibody
    • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 antibody
    • LILRB1 antibody
    • LIR 1 antibody
    • LIR-1 antibody
    • LIR1 antibody
    • LIRB1_HUMAN antibody
    • MIR 7 antibody
    • MIR-7 antibody
    • MIR7 antibody
    • Monocyte/macrophage immunoglobulin like receptor 7 antibody
    • Monocyte/macrophage immunoglobulin-like receptor 7 antibody
    • PIR B antibody
    • PIRB antibody
    see all

Images

  • All lanes : Anti-LILRB1 antibody (ab237625) at 1/500 dilution

    Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 2 : Rat brain tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Paraffin-embedded human tonsil tissue stained for LILRB1 with ab237625 at a 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HEK-293 (human epithelial cell line from embryonic kidney) cells stained for LILRB1 using ab237625 at a dilution of 1/133 in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

References

ab237625 has not yet been referenced specifically in any publications.

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