Overview

  • Product name

    Anti-LILRB1 antibody [EPR11256]
    See all LILRB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11256] to LILRB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LILRB1 aa 600 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8NHL6

  • Positive control

    • WB: THP-1, A431 and Raji lysates. IHC-P: human tonsil tissue. ICC/IF: Jurkat cells. FC: THP-1 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11256
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 71 kDa.

For unpurified use at  1/1000 - 1/5000

IHC-P 1/2000.

Antigen retrieval is recommended. 

For unpurified use at  1/250 - 1/500

See IHC antigen retrieval protocols.

ICC/IF 1/100 - 1/250.
Flow Cyt 1/150.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
    • Tissue specificity

      Expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells.
    • Sequence similarities

      Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD85 antibody
      • CD85 antigen antibody
      • CD85 antigen like family member J antibody
      • CD85 antigen-like family member J antibody
      • CD85j antibody
      • CD85j antigen antibody
      • Ig like transcript 2 antibody
      • ILT 2 antibody
      • ILT-2 antibody
      • ILT2 antibody
      • immunoglobulin like transcript 2 antibody
      • Immunoglobulin-like transcript 2 antibody
      • leukocyte Ig like receptor 1 antibody
      • Leukocyte immunoglobulin like receptor 1 antibody
      • leukocyte immunoglobulin like receptor subfamily B member 1 antibody
      • Leukocyte immunoglobulin like receptor subfamily B member 1 precursor antibody
      • leukocyte immunoglobulin like receptor subfamily B member 1 soluble isoform antibody
      • leukocyte immunoglobulin like receptor, subfamily B, member 1 antibody
      • Leukocyte immunoglobulin-like receptor 1 antibody
      • Leukocyte immunoglobulin-like receptor subfamily B member 1 antibody
      • leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform antibody
      • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 antibody
      • LILRB1 antibody
      • LIR 1 antibody
      • LIR-1 antibody
      • LIR1 antibody
      • LIRB1_HUMAN antibody
      • MIR 7 antibody
      • MIR-7 antibody
      • MIR7 antibody
      • Monocyte/macrophage immunoglobulin like receptor 7 antibody
      • Monocyte/macrophage immunoglobulin-like receptor 7 antibody
      • PIR B antibody
      • PIRB antibody
      see all

    Images

    • Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling LILRB1 with purified ab170909 at 1/150 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • All lanes : Anti-LILRB1 antibody [EPR11256] (ab170909) at 1/10000 dilution (Purified)

      Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
      Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 71 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling LILRB1 with purified ab170909 at 1/250 dilution (5.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling LILRB1 with purified ab170909 at 1/2000 dilution (0.73 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • All lanes : Anti-LILRB1 antibody [EPR11256] (ab170909) at 1/1000 dilution ((unpurified))

      Lane 1 : A431 cell lysate
      Lane 2 : THP-1 cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 71 kDa

    • Immunofluorescent analysis of Jurkat cells labeling LILRB1 using unpurified ab170909 at 1/100 dilution (green). DAPI nuclear staining (blue).

    • Immunohistochemical analysis of paraffin-embedded Human tonsil  tissue labeling LILRB1 using unpurified ab170909 at 1/250 dilution.  

    References

    This product has been referenced in:

    • Wan R  et al. Human Leukocyte Antigen-G Inhibits the Anti-Tumor Effect of Natural Killer Cells via Immunoglobulin-Like Transcript 2 in Gastric Cancer. Cell Physiol Biochem 44:1828-1841 (2017). Read more (PubMed: 29224003) »
    See 1 Publication for this product

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