Recombinant
RabMAb

Anti-LILRB1 antibody [EPR11256] (ab170909)

Overview

  • Product name
    Anti-LILRB1 antibody [EPR11256]
    See all LILRB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11256] to LILRB1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LILRB1 aa 600 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8NHL6

  • Positive control
    • A431, THP-1 and Raji cell lysates; Human tonsil tissue; Jurkat cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11256
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 71 kDa.
IHC-P 1/250 - 1/500.

Antigen retrieval is recommended.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
    • Tissue specificity
      Expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells.
    • Sequence similarities
      Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain
      Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD85 antibody
      • CD85 antigen antibody
      • CD85 antigen like family member J antibody
      • CD85 antigen-like family member J antibody
      • CD85j antibody
      • CD85j antigen antibody
      • Ig like transcript 2 antibody
      • ILT 2 antibody
      • ILT-2 antibody
      • ILT2 antibody
      • immunoglobulin like transcript 2 antibody
      • Immunoglobulin-like transcript 2 antibody
      • leukocyte Ig like receptor 1 antibody
      • Leukocyte immunoglobulin like receptor 1 antibody
      • leukocyte immunoglobulin like receptor subfamily B member 1 antibody
      • Leukocyte immunoglobulin like receptor subfamily B member 1 precursor antibody
      • leukocyte immunoglobulin like receptor subfamily B member 1 soluble isoform antibody
      • leukocyte immunoglobulin like receptor, subfamily B, member 1 antibody
      • Leukocyte immunoglobulin-like receptor 1 antibody
      • Leukocyte immunoglobulin-like receptor subfamily B member 1 antibody
      • leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform antibody
      • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 antibody
      • LILRB1 antibody
      • LIR 1 antibody
      • LIR-1 antibody
      • LIR1 antibody
      • LIRB1_HUMAN antibody
      • MIR 7 antibody
      • MIR-7 antibody
      • MIR7 antibody
      • Monocyte/macrophage immunoglobulin like receptor 7 antibody
      • Monocyte/macrophage immunoglobulin-like receptor 7 antibody
      • PIR B antibody
      • PIRB antibody
      see all

    Images

    • Immunofluorescent analysis of Jurkat cells labeling LILRB1 using ab170909 at 1/100 dilution (green). DAPI nuclear staining (blue).

    • All lanes : Anti-LILRB1 antibody [EPR11256] (ab170909) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : THP-1 cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 71 kDa

    • Immunohistochemical analysis of paraffin-embedded Human tonsil  tissue labeling LILRB1 using ab170909 at 1/250 dilution.  

    References

    ab170909 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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