Recombinant
RabMAb

Recombinant Anti-LILRB1 antibody [EPR21007] - BSA and Azide free (ab229853)

Overview

  • Product name

    Anti-LILRB1 antibody [EPR21007] - BSA and Azide free
    See all LILRB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR21007] to LILRB1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LILRB1 aa 350-450. The exact sequence is proprietary.
    Database link: Q8NHL6

  • Positive control

    • IHC-P: Human NK/T-cell lymphoma tissue.
  • General notes

    Ab229853 is the carrier-free version of ab229186. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab229853 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21007
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 90-110 kDa (predicted molecular weight: 71 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
  • Tissue specificity

    Expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells.
  • Sequence similarities

    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD85 antibody
    • CD85 antigen antibody
    • CD85 antigen like family member J antibody
    • CD85 antigen-like family member J antibody
    • CD85j antibody
    • CD85j antigen antibody
    • Ig like transcript 2 antibody
    • ILT 2 antibody
    • ILT-2 antibody
    • ILT2 antibody
    • immunoglobulin like transcript 2 antibody
    • Immunoglobulin-like transcript 2 antibody
    • leukocyte Ig like receptor 1 antibody
    • Leukocyte immunoglobulin like receptor 1 antibody
    • leukocyte immunoglobulin like receptor subfamily B member 1 antibody
    • Leukocyte immunoglobulin like receptor subfamily B member 1 precursor antibody
    • leukocyte immunoglobulin like receptor subfamily B member 1 soluble isoform antibody
    • leukocyte immunoglobulin like receptor, subfamily B, member 1 antibody
    • Leukocyte immunoglobulin-like receptor 1 antibody
    • Leukocyte immunoglobulin-like receptor subfamily B member 1 antibody
    • leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform antibody
    • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 antibody
    • LILRB1 antibody
    • LIR 1 antibody
    • LIR-1 antibody
    • LIR1 antibody
    • LIRB1_HUMAN antibody
    • MIR 7 antibody
    • MIR-7 antibody
    • MIR7 antibody
    • Monocyte/macrophage immunoglobulin like receptor 7 antibody
    • Monocyte/macrophage immunoglobulin-like receptor 7 antibody
    • PIR B antibody
    • PIRB antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling LILRB1 with ab229186 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining in tumor-infiltrating immunocytes is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229186).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LILRB1 with ab229186 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining in Kupffer cells of human liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229186).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue labeling LILRB1 with ab229186 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining in human NK/T-cell lymphoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229186).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

References

ab229853 has not yet been referenced specifically in any publications.

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