Recombinant
RabMAb

Recombinant Anti-LILRB1 antibody [EPR22861-6] (ab238145)

Overview

  • Product name

    Anti-LILRB1 antibody [EPR22861-6]
    See all LILRB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22861-6] to LILRB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human LILRB1 aa 1-500. The exact sequence is proprietary.
    Database link: Q8NHL6

  • Positive control

    • WB: IM-9 whole cell lysate. ICC/IF: Human PBMC cells. Flow Cyt: Human PBMC and HEK-293T cells. IP: IM-9 cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22861-6
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 71 kDa.
ICC 1/50.
Flow Cyt 1/500.
IP 1/30.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
    • Tissue specificity

      Expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells.
    • Sequence similarities

      Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD85 antibody
      • CD85 antigen antibody
      • CD85 antigen like family member J antibody
      • CD85 antigen-like family member J antibody
      • CD85j antibody
      • CD85j antigen antibody
      • Ig like transcript 2 antibody
      • ILT 2 antibody
      • ILT-2 antibody
      • ILT2 antibody
      • immunoglobulin like transcript 2 antibody
      • Immunoglobulin-like transcript 2 antibody
      • leukocyte Ig like receptor 1 antibody
      • Leukocyte immunoglobulin like receptor 1 antibody
      • leukocyte immunoglobulin like receptor subfamily B member 1 antibody
      • Leukocyte immunoglobulin like receptor subfamily B member 1 precursor antibody
      • leukocyte immunoglobulin like receptor subfamily B member 1 soluble isoform antibody
      • leukocyte immunoglobulin like receptor, subfamily B, member 1 antibody
      • Leukocyte immunoglobulin-like receptor 1 antibody
      • Leukocyte immunoglobulin-like receptor subfamily B member 1 antibody
      • leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform antibody
      • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 antibody
      • LILRB1 antibody
      • LIR 1 antibody
      • LIR-1 antibody
      • LIR1 antibody
      • LIRB1_HUMAN antibody
      • MIR 7 antibody
      • MIR-7 antibody
      • MIR7 antibody
      • Monocyte/macrophage immunoglobulin like receptor 7 antibody
      • Monocyte/macrophage immunoglobulin-like receptor 7 antibody
      • PIR B antibody
      • PIRB antibody
      see all

    Images

    • All lanes : Anti-LILRB1 antibody [EPR22861-6] (ab238145) at 1/1000 dilution

      Lane 1 : IM-9 (human multiple myeloma b lymphoblast) whole cell lysate
      Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 71 kDa
      Observed band size: 90-110 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 22844324).

      Negative control: K-562 (PMID: 22844324).

    • LILRB1 was immunoprecipitated from 0.35 mg IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate with ab238145 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238145 at a 1/1000 dilution (0.48 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

      Lane 1: IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10μg
      Lane 2: ab238145 IP in IM-9 whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238145 in IM-9 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling LILRB1 with ab238145 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of human PBMC is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with myc-tagged LILRB2 expression vector (Left) / HEK-293T transfected with myc-tagged LILRB1 expression vector (Right) cells labeling LILRB1 with ab238145 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG (black) or ab238145 (red). Then fixed with 2% PFA followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647. Gated on myc+ population.

    • Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling LILRB1 with ab238145 at 1/500 dilution (0.1μg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with anti-CD11b conjugated to BV421. Then stained with rabbit IgG (Left) or ab238145 (Right). Gated on viable cells.

    References

    ab238145 has not yet been referenced specifically in any publications.

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