Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22861-6] to LILRB1
- Suitable for: WB, Flow Cyt, IP, ICC/IF
- Reacts with: Human
Product nameAnti-LILRB1 antibody [EPR22861-6]
See all LILRB1 primary antibodies
DescriptionRabbit monoclonal [EPR22861-6] to LILRB1
Tested applicationsSuitable for: WB, Flow Cyt, IP, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human LILRB1 aa 1-500. The exact sequence is proprietary.
Database link: Q8NHL6
- WB: IM-9 whole cell lysate. ICC/IF: Human PBMC cells. Flow Cyt: Human PBMC and HEK-293T cells. IP: IM-9 cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab238145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 71 kDa.|
FunctionReceptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Tissue specificityExpressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells.
Sequence similaritiesContains 4 Ig-like C2-type (immunoglobulin-like) domains.
DomainContains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsPhosphorylated on tyrosine residues. Dephosphorylated by PTPN6.
- Information by UniProt
- CD85 antibody
- CD85 antigen antibody
- CD85 antigen like family member J antibody
All lanes : Anti-LILRB1 antibody [EPR22861-6] (ab238145) at 1/1000 dilution
Lane 1 : IM-9 (human multiple myeloma b lymphoblast) whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 71 kDa
Observed band size: 90-110 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 22844324).
Negative control: K-562 (PMID: 22844324).
LILRB1 was immunoprecipitated from 0.35 mg IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate with ab238145 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238145 at a 1/1000 dilution (0.48 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10μg
Lane 2: ab238145 IP in IM-9 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238145 in IM-9 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling LILRB1 with ab238145 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of human PBMC is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with myc-tagged LILRB2 expression vector (Left) / HEK-293T transfected with myc-tagged LILRB1 expression vector (Right) cells labeling LILRB1 with ab238145 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG (black) or ab238145 (red). Then fixed with 2% PFA followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647. Gated on myc+ population.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling LILRB1 with ab238145 at 1/500 dilution (0.1μg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with anti-CD11b conjugated to BV421. Then stained with rabbit IgG (Left) or ab238145 (Right). Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238145 has not yet been referenced specifically in any publications.