PE Anti-LILRB1 antibody [GHI/75], prediluted (ab186074)
Key features and details
- PE Mouse monoclonal [GHI/75] to LILRB1, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
PE Anti-LILRB1 antibody [GHI/75], prediluted
See all LILRB1 primary antibodies -
Description
PE Mouse monoclonal [GHI/75] to LILRB1, prediluted -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human LILRB1. Hairy cell leukaemia cells.
Database link: Q8NHL6 -
Positive control
- Flow Cyt: Human blood cells.
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General notes
ab186074 recognizes LILRB1, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
GHI/75 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Target
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Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. -
Tissue specificity
Expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells. -
Sequence similarities
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylated on tyrosine residues. Dephosphorylated by PTPN6. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10859 Human
- Omim: 604811 Human
- SwissProt: Q8NHL6 Human
- Unigene: 667388 Human
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Alternative names
- CD85 antibody
- CD85 antigen antibody
- CD85 antigen like family member J antibody
see all
Images
Datasheets and documents
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Datasheet download
References (1)
ab186074 has been referenced in 1 publication.
- Albayati Z et al. The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets. Scand J Immunol 86:396-407 (2017). PubMed: 28817184