Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase.
Our Abpromise guarantee covers the use of ab10561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).|
FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
Cellular localizationCytoplasm. Cell projection > growth cone.
- Information by UniProt
- EC 188.8.131.52 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
Rabbit polyclonal to LIM kinase (1/500) - ab10561.
18 ug of mouse brain lysate loaded.
Expected mol weight : 73 kD. Bands observed at 75 kD which we believe represents LIM kinase (doublet - may indicate modification such as phosphorylation) and 25 kD (we do not know the identity of this band).
Secondary ab - Goat polyclonal to rabbit IgG (HRP) preabsorbedRabbit polyclonal to LIM kinase (1/500) - ab10561. 18 ug of mouse brain lysate loaded. Expected mol weight : 73 kD. Bands observed at 75 kD which we believe represents LIM kinase (doublet - may indicate modification such as phosphorylation) and 25 kD (we do not know the identity of this band). Secondary ab - Goat polyclonal to rabbit IgG (HRP) preabsorbed
ab10561 has not yet been referenced specifically in any publications.