Product nameAnti-LIM Kinase 1 antibody
See all LIM Kinase 1 primary antibodies
DescriptionRabbit polyclonal to LIM Kinase 1
Specificityab39641 detects endogenous levels of total LIM Kinase 1 protein.
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide (non-phosphorylated) derived from human LIM Kinase 1 around the phosphorylation site of Threonine 508.
- A2780 and NIH/3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab39641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100 (PMID 19208621).
WB: 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
Cellular localizationCytoplasm. Cell projection > growth cone.
- Information by UniProt
- EC 184.108.40.206 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
All lanes : Anti-LIM Kinase 1 antibody (ab39641) at 1/500 dilution
Lane 1 : NIH/3T3 cell extracts
Lane 2 : A2780 cell extracts
Predicted band size: 73 kDa
Observed band size: 73 kDa
5-30µg of cell extract was loaded per lane.
ab39641 staining LIM Kinase 1 in the growth cones of Human DRG neurons by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature and permeabilized (0.1% Triton X-100 and 1× phosphate-buffered saline). After blocking, cells were incubated for double staining with primary antibodies, followed by incubation with the appropriate Cy3-secondary antibody for 30 minutes and with 4',6-diamidino-2-phenylindole. F-actin staining with Alexa 488-phalloidin.
Scale bar = 10µm.
This product has been referenced in:
- Zhou Y et al. DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion. Oncol Rep 29:605-12 (2013). Read more (PubMed: 23233092) »
- Tondeleir D et al. Cells lacking ß-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics : (2012). Read more (PubMed: 22448045) »