Anti-LIM Kinase 1 antibody (ab39641)
Key features and details
- Rabbit polyclonal to LIM Kinase 1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LIM Kinase 1 antibody
See all LIM Kinase 1 primary antibodies -
Description
Rabbit polyclonal to LIM Kinase 1 -
Host species
Rabbit -
Specificity
ab39641 detects endogenous levels of total LIM Kinase 1 protein. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide (non-phosphorylated) derived from human LIM Kinase 1 around the phosphorylation site of Threonine 508.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Human
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| All applications |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
| Notes |
|---|
ICC/IF: 1/100 (PMID 19208621).
WB: 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. -
Tissue specificity
Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. -
Involvement in disease
Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. -
Cellular localization
Cytoplasm. Cell projection > growth cone. - Information by UniProt
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Database links
- Entrez Gene: 3984 Human
- Entrez Gene: 16885 Mouse
- Entrez Gene: 65172 Rat
- Omim: 601329 Human
- SwissProt: P53667 Human
- SwissProt: P53668 Mouse
- SwissProt: P53669 Rat
- Unigene: 647035 Human
see all -
Alternative names
- EC 2.7.1.37 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
see all
Images
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Western blot - Anti-LIM Kinase 1 antibody (ab39641)All lanes : Anti-LIM Kinase 1 antibody (ab39641) at 1/500 dilution
Lane 1 : NIH/3T3 cell extracts
Lane 2 : A2780 cell extracts
Predicted band size: 73 kDa
Observed band size: 73 kDa
5-30µg of cell extract was loaded per lane.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab39641 has been referenced in 3 publications.
- Zhou Y et al. DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion. Oncol Rep 29:605-12 (2013). PubMed: 23233092
- Tondeleir D et al. Cells lacking ß-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics : (2012). PubMed: 22448045
- Montani L et al. Neuronal Nogo-A Modulates Growth Cone Motility via Rho-GTP/LIMK1/Cofilin in the Unlesioned Adult Nervous System. J Biol Chem 284:10793-807 (2009). WB, ICC/IF ; Rat . PubMed: 19208621