For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    lim-kinase-1-antibody-ab39641.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Neural Signal Transduction
Share by email

Anti-LIM Kinase 1 antibody (ab39641)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-LIM Kinase 1 antibody (ab39641)

    Key features and details

    • Rabbit polyclonal to LIM Kinase 1
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant Human LIM Kinase 1 protein (ab70186)

    View more associated products

    Overview

    • Product name

      Anti-LIM Kinase 1 antibody
      See all LIM Kinase 1 primary antibodies
    • Description

      Rabbit polyclonal to LIM Kinase 1
    • Host species

      Rabbit
    • Specificity

      ab39641 detects endogenous levels of total LIM Kinase 1 protein.
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide (non-phosphorylated) derived from human LIM Kinase 1 around the phosphorylation site of Threonine 508.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • LIM
      • Cancer
      • Signal transduction
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human LIM Kinase 1 protein (ab70186)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab39641 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Human
    All applications
    Rat
    Application Abreviews Notes
    WB
    Notes
  • Application notes
    ELISA: 1/4000.
    ICC/IF: 1/100 (PMID 19208621).
    WB: 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
    • Tissue specificity

      Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
    • Involvement in disease

      Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 2 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated.
      Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
      Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
    • Cellular localization

      Cytoplasm. Cell projection > growth cone.
    • Target information above from: UniProt accession P53667 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3984 Human
      • Entrez Gene: 16885 Mouse
      • Entrez Gene: 65172 Rat
      • Omim: 601329 Human
      • SwissProt: P53667 Human
      • SwissProt: P53668 Mouse
      • SwissProt: P53669 Rat
      • Unigene: 647035 Human
      • Unigene: 15409 Mouse
      • Unigene: 11250 Rat
      see all
    • Alternative names

      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM kinase antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-LIM Kinase 1 antibody (ab39641)
      Western blot - Anti-LIM Kinase 1 antibody (ab39641)
      All lanes : Anti-LIM Kinase 1 antibody (ab39641) at 1/500 dilution

      Lane 1 : NIH/3T3 cell extracts
      Lane 2 : A2780 cell extracts

      Predicted band size: 73 kDa
      Observed band size: 73 kDa



      5-30µg of cell extract was loaded per lane.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab39641? Please let us know so that we can cite the reference in this datasheet.

    ab39641 has been referenced in 3 publications.

    • Zhou Y  et al. DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion. Oncol Rep 29:605-12 (2013). PubMed: 23233092
    • Tondeleir D  et al. Cells lacking ß-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics : (2012). PubMed: 22448045
    • Montani L  et al. Neuronal Nogo-A Modulates Growth Cone Motility via Rho-GTP/LIMK1/Cofilin in the Unlesioned Adult Nervous System. J Biol Chem 284:10793-807 (2009). WB, ICC/IF ; Rat . PubMed: 19208621

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I'm desperate for a LIMK1 antibody for use in Drosophila, but have been unable to find a product that has been tested in flies. Looking at the fly LIMK1 sequence, the region around the phosphorylation site is fairly well conserved. So: 1) Could you tell me the precise sequence used as the antigen for ab38508 and ab39641? 2) If the sequence is not 100% conserved but is highly similar, I would like to test the antibodies. Is there any way that I could be sent a small sample of the antibodies to try in advance? I would be happy to provide you with any positive data that could be used on the website - I'm sure there will be plenty of other fly-people out there that are prepared to pay for Drosophila-tested products! Many thanks, PS - I would test the antibody by western blot on lysates from wild-type flies, and from flies with heat shock-induced RNAi and overexpression. I would also test the antibody by immunofluorescence in whole-mount brains. I would test the phospho-antibody by overexpressing PAK by heatshock (for Western) or in specific tissues (for immunofluorescence).

    Read More

    Abcam community

    Verified customer

    Asked on Dec 13 2006

    Answer

    Thank you for your enquiry. Following a sequence alignment; http://www.ebi.ac.uk/cgi-bin/jobresults/clustalw/clustalw-20061214-15265852.aln? It appears that there is a degree of sequence similarity between the Drosophila and Human homologs. However, around the phospho T508 site there is 100% sequence similarity towards the C terminal region but some sequence divergence towards the N terminal region. I consider it likely to work however, given the run of residues that are identical around the threonine phospho site. Unfortunately the precise immunogen sequence is not known as this information is considered proprietary by the source of this antibody. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If the antibody is being used in an untested species or application, we cannot offer a replacement or refund; however, we invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for discounts on future antibody purchases or rewards such as Amazon.com gift certificates. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 14 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.