Anti-LIM Kinase 1 antibody (ab39641)

Submit a review Q&A (1)References (3)

Overview

  • Product name

    Anti-LIM Kinase 1 antibody
    See all LIM Kinase 1 primary antibodies

  • Description

    Rabbit polyclonal to LIM Kinase 1

  • Host species

    Rabbit

  • Specificity

    ab39641 detects endogenous levels of total LIM Kinase 1 protein.

  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide (non-phosphorylated) derived from human LIM Kinase 1 around the phosphorylation site of Threonine 508.

  • Positive control

    • A2780 and NIH/3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab39641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notes
    ELISA: 1/4000.
    ICC/IF: 1/100 (PMID 19208621).
    WB: 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.

    • Tissue specificity

      Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

    • Involvement in disease

      Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 2 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Autophosphorylated.
      Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
      Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.

    • Cellular localization

      Cytoplasm. Cell projection > growth cone.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM kinase antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-LIM Kinase 1 antibody (ab39641)
      Western blot - Anti-LIM Kinase 1 antibody (ab39641)
      All lanes : Anti-LIM Kinase 1 antibody (ab39641) at 1/500 dilution

      Lane 1 : NIH/3T3 cell extracts
      Lane 2 : A2780 cell extracts

      Predicted band size: 73 kDa
      Observed band size: 73 kDa



      5-30µg of cell extract was loaded per lane.
    • Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody (ab39641)
      Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody (ab39641)Image from Montani L et al, J Biol Chem. 2009 Apr 17;284(16):10793-807. Epub 2009 Feb 9, Fig 5, DOI 10.1074/jbc.M808297200.
      ab39641 staining LIM Kinase 1 in the growth cones of Human DRG neurons by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature and permeabilized (0.1% Triton X-100 and 1× phosphate-buffered saline). After blocking, cells were incubated for double staining with primary antibodies, followed by incubation with the appropriate Cy3-secondary antibody for 30 minutes and with 4',6-diamidino-2-phenylindole. F-actin staining with Alexa 488-phalloidin.

      Scale bar = 10µm.

    References

    This product has been referenced in:

    • Zhou Y  et al. DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion. Oncol Rep 29:605-12 (2013). Read more (PubMed: 23233092) »
    • Tondeleir D  et al. Cells lacking ß-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics : (2012). Read more (PubMed: 22448045) »
    See all 3 Publications for this product

    Publishing research using ab39641? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Question

    I'm desperate for a LIMK1 antibody for use in Drosophila, but have been unable to find a product that has been tested in flies. Looking at the fly LIMK1 sequence, the region around the phosphorylation site is fairly well conserved. So: 1) Could you tell me the precise sequence used as the antigen for ab38508 and ab39641? 2) If the sequence is not 100% conserved but is highly similar, I would like to test the antibodies. Is there any way that I could be sent a small sample of the antibodies to try in advance? I would be happy to provide you with any positive data that could be used on the website - I'm sure there will be plenty of other fly-people out there that are prepared to pay for Drosophila-tested products! Many thanks, PS - I would test the antibody by western blot on lysates from wild-type flies, and from flies with heat shock-induced RNAi and overexpression. I would also test the antibody by immunofluorescence in whole-mount brains. I would test the phospho-antibody by overexpressing PAK by heatshock (for Western) or in specific tissues (for immunofluorescence).

    Read More
    Answer

    Thank you for your enquiry. Following a sequence alignment; http://www.ebi.ac.uk/cgi-bin/jobresults/clustalw/clustalw-20061214-15265852.aln? It appears that there is a degree of sequence similarity between the Drosophila and Human homologs. However, around the phospho T508 site there is 100% sequence similarity towards the C terminal region but some sequence divergence towards the N terminal region. I consider it likely to work however, given the run of residues that are identical around the threonine phospho site. Unfortunately the precise immunogen sequence is not known as this information is considered proprietary by the source of this antibody. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If the antibody is being used in an untested species or application, we cannot offer a replacement or refund; however, we invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for discounts on future antibody purchases or rewards such as Amazon.com gift certificates. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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